Antibody:	Ads-1 antibody
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS28: early nephron	Present	Regionally restricted	5A	Expression is in cells at the boundary of Bowman's capsule.
TS28: cortical collecting duct	Present		5A
TS28: distal straight tubule of renal cortex	Present		5A
TS28: segment 1 of proximal tubule	Present		5A

Structure	Level	Pattern	Image	Note
TS28: premacula segment of distal straight tubule of inner stripe of inner medulla	Present		5B

Structure	Level	Pattern	Image	Note
TS28: thin descending limb of inner medulla	Present	Regionally restricted	5C	Expression extends from the boundary of the S3 segment of the proximal tubule through the entire length of the loop.

Structure	Level	Pattern	Image	Note
TS28: cortical collecting duct	Strong	Regionally restricted	5D	Expression is in principal cells, absent in intercalated cells.

Structure	Level	Pattern	Image	Note
TS28: papillary duct	Absent
TS28: renal vasculature	Absent
TS28: small intestine lamina propria	Absent
TS28: ureter	Absent

Structure	Level	Pattern	Image	Note
TS28: small intestine crypt of lieberkuhn	Present		9A
TS28: intestinal villus	Present	Regionally restricted	9A	Expression is in the upper half of the villus in the epithelial cells, absent in basement membrane.
TS28: small intestine lamina propria	Absent		9A
TS28: small intestine muscularis mucosa	Absent		9A

Structure	Level	Pattern	Image	Note
TS28: intestinal villus	Present		9C

Structure	Level	Pattern	Image	Note
TS28: colon epithelium	Strong		10A	Highest levels were at the crown of the villus.