Summary | Mutation origin | Mutation description | Expression | Recombinase activity | Phenotypes | Disease models | Find Mice (IMSR) | References

Symbol:	Slc32a1tm2(cre)Lowl
Name:	solute carrier family 32 (GABA vesicular transporter), member 1; targeted mutation 2, Bradford B Lowell
MGI ID:	MGI:5141270
Synonyms:	VGAT-Cre, vGATiresCre, Vgat-ires-Cre
Gene:	Slc32a1  Location: Chr2:158452687-158457668 bp, + strand  Genetic Position: Chr2, 78.72 cM, cytoband H1-3
Alliance:	Slc32a1tm2(cre)Lowl page

Germline Transmission:	Earliest citation of germline transmission: J:174691
Parent Cell Line:	W4 (ES Cell)
Strain of Origin:	129S6/SvEvTac

Allele Type:    Targeted (Recombinase) Mutation:    Insertion   Slc32a1tm2(cre)Lowl expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Slc32a1 (MGI:1194488)   Mutation details: A targeting vector, constructed from a BAC clone containing the Slc32a1 (Vgat) gene, was utilized to insert an internal ribosomal entry site followed by the cre recombinase sequence into the Slc32a1 locus just downstream of the endogenous stop codon. Cre is expressed from the Slc32a1 gene promoter. Immunohistochemistry on brain and Western blot analysis shows decreased expression of SLC32A1 protein in homozygotes. (J:174691, J:308516)

Activity:	Tissue activity of this recombinase allele
Driver:	Slc32a1 (mouse) Summary of all recombinase alleles driven by Slc32a1. Comparative matrix view of recombinase activities Alliance mouse Slc32a1 gene page

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:

208072 assay results 4 RNA-Seq or microarray experiment(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Slc32a1 Mutation:	55 strains or lines available

Original:	J:174691 Vong L, et al., Leptin Action on GABAergic Neurons Prevents Obesity and Reduces Inhibitory Tone to POMC Neurons. Neuron. 2011 Jul 14;71(1):142-54
All:	422 reference(s)