Probe:	Ifrg15 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component mesenchyme	Present	Diffuse	1A	Diffuse expression in epithelial and mesenchymal tissues in the mandible.
TS17: oral epithelium	Present	Diffuse	1A	Diffuse expression in epithelial and mesenchymal tissues in the mandible.

Structure	Level	Pattern	Image	Note
TS20: mandibular process mesenchyme	Present	Regionally restricted	1B	Expresssion in the thickened epithelium and underlying mesenchyme.
TS20: oral epithelium	Present	Regionally restricted	1B	Expresssion in the thickened epithelium and underlying mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: lower jaw molar epithelium	Present	Regionally restricted	1C	Expression in the budding epithelium.
TS22: lower jaw molar mesenchyme	Present	Regionally restricted	1C	Expression in surrounding mesenchyme that consists of focally condensed cells.

Structure	Level	Pattern	Image	Note
TS23: lower jaw molar enamel organ	Present	Regionally restricted	1D	Expression in both inner and outer epithelial cells of the enamel organ, including the area of the primary enamel knot.
TS23: inner enamel epithelium	Present		1D
TS23: outer enamel epithelium	Present		1D
TS23: primary enamel knot	Present		1D

Structure	Level	Pattern	Image	Note
TS23: primary enamel knot	Present	Regionally restricted	1E	Expression in the cytoplasm of cells in the primary enamel knot.
TS23: lower jaw molar dental papilla	Weak	Regionally restricted	1E	Faint expression in mesenchymal cells condensing to the enamel organ.

Structure	Level	Pattern	Image	Note
TS24: cervical loop	Strong	Regionally restricted	1F	Strong expression in the buccal and lingual cervical loops of the invaginating enamel organ. Expression restricted to inner and outer enamel epithelial cells and the stratum intermedium
TS24: inner enamel epithelium	Present		1F
TS24: outer enamel epithelium	Present		1F
TS24: stratum intermedium	Present		1F
TS24: lower jaw molar dental papilla	Present		1F

Structure	Level	Pattern	Image	Note
TS24: inner enamel epithelium	Present	Regionally restricted	1G	Expression within the cytoplasm of inner enamel epithelium.
TS24: lower jaw molar dental papilla	Weak	Regionally restricted	1G	Faint expression in the underlying mesenchymal cells in the dental papilla.

Structure	Level	Pattern	Image	Note
TS25: lower jaw molar enamel organ	Present	Regionally restricted	1H
TS25: inner enamel epithelium	Strong		1H	Strong expression restricted to the inner enamel epithelium and adjacent stratum intermedium.
TS25: stratum intermedium	Strong		1H	Strong expression restricted to the inner enamel epithelium and adjacent stratum intermedium.

Structure	Level	Pattern	Image	Note
TS26: lower jaw molar enamel organ	Present	Regionally restricted	1I
TS26: inner enamel epithelium	Strong		1I	Strong expression restricted to the inner enamel epithelium and adjacent stratum intermedium.
TS26: stratum intermedium	Strong		1I	Strong expression restricted to the inner enamel epithelium and adjacent stratum intermedium.

Structure	Level	Pattern	Image	Note
TS26: lower jaw molar enamel organ	Present	Regionally restricted	1J
TS26: inner enamel epithelium	Present	Regionally restricted	1J	Expression within the cytoplams of the inner enamel epithelium and stratum intermedium.
TS26: stratum intermedium	Present	Regionally restricted	1J	Expression within the cytoplams of the inner enamel epithelium and stratum intermedium.

Structure	Level	Pattern	Image	Note
TS27: lower jaw molar	Weak	Regionally restricted	1K	Expression localized to ameloblasts and odontoblasts, markedly weaker than the restricted signals at E17.0 and E18.0
TS27: enamel organ	Present	Regionally restricted	1K	Expression in ameloblasts.
TS27: dental papilla	Present	Regionally restricted	1K	Expression in odontoblasts. Also observed in mesenchymal cells of both the buccal and lingual cusp sites in the dental papilla.

Structure	Level	Pattern	Image	Note
TS27: lower jaw molar	Weak	Regionally restricted	1L	Expression localized to ameloblasts and odontoblasts, markedly weaker than the restricted signals at E17.0 and E18.0
TS27: enamel organ	Present	Regionally restricted	1L	Expression in ameloblasts.
TS27: dental papilla	Present	Regionally restricted	1L	Expression in odontoblasts. Also observed in mesenchymal cells of both the buccal and lingual cusp sites in the dental papilla.

Structure	Level	Pattern	Image	Note
TS27: dental papilla	Present	Regionally restricted	1M	Expression noted within the cytoplasm of ameloblasts and odontoblasts.
TS27: enamel organ	Present	Regionally restricted	1M	Expression noted within the cytoplasm of ameloblasts and odontoblasts.

Structure	Level	Pattern	Image	Note
TS27: lower jaw molar	Weak	Regionally restricted	1N	Expression localized to ameloblasts and odontoblasts, markedly weaker than the restricted signals at E17.0 and E18.0
TS27: enamel organ	Present	Regionally restricted	1N	Expression in ameloblasts.
TS27: dental papilla	Present	Regionally restricted	1N	Expression in odontoblasts. Also observed in mesenchymal cells of both the buccal and lingual cusp sites in the dental papilla.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted	2A	Expression in cells of neuroepithelial cells of the lateral ventricles .
TS22: olfactory epithelium	Present		2A

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted	2B	Expression within the cytoplasm of neuroepithelial cells of the lateral ventricle.

Structure	Level	Pattern	Image	Note
TS22: retina	Present		2C

Structure	Level	Pattern	Image	Note
TS27: lacrimal gland	Present		2D

Structure	Level	Pattern	Image	Note
TS27: epidermis	Present		2E
TS27: hair follicle	Present		2E

Structure	Level	Pattern	Image	Note
TS24: tongue muscle	Present		2F
TS24: sublingual gland	Present	Regionally restricted	2F	Expression in cells of the sublingual duct.

Structure	Level	Pattern	Image	Note
TS24: tongue muscle	Present	Regionally restricted	2G	Expression within the cytoplasm of muscle cells.

Structure	Level	Pattern	Image	Note
TS24: sublingual gland	Present	Regionally restricted	2H	Expression with the cytoplasm of sublingual duct cells.

Structure	Level	Pattern	Image	Note
TS24: viscerocranium	Present	Regionally restricted	2I	Expression in osteoblasts of membranous bone.
TS24: Meckel's cartilage	Present	Regionally restricted	2I	Expression in chondrocytes of Meckel's cartilage.