Gene Expression Data
Assay Details
Assay
|
Reference:
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J:31260
Wuenschell CW, et al., Embryonic mouse lung epithelial progenitor cells co-express immunohistochemical markers of diverse mature cell lineages. J Histochem Cytochem. 1996 Feb;44(2):113-23
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5286165
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Gene symbol:
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Calca
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Gene name:
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calcitonin/calcitonin-related polypeptide, alpha
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Antibody:
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anti-CGRP
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
not shown:
embryonic day 13.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 13.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: lung epithelium |
Present |
Regionally restricted |
|
Expression in epithelium of smaller airways; no expression in epithelium of larger airways.
|
Specimen
1A:
embryonic day 14.0
(more )
Specimen
1A:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 14.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: lung epithelium |
Present |
Regionally restricted |
1A
|
Expression in epithelium of smaller, more peripheral airways; expression tended to be continuous, strongest at luminal edge. Little or no expression in epithelium of larger proximal airways.
|
Specimen
1D:
embryonic day 14.0
(more )
Specimen
1D:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 14.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: lung epithelium |
Present |
Regionally restricted |
1D
|
Expression confined to epithelium of small airways; maximal level of expression not as high as at E14.
|
Specimen
not shown:
embryonic day 15.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 15.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: lung epithelium |
Present |
Regionally restricted |
|
Expression confined to epithelium of small airways; maximal level of expression not as high as at E14.
|
Specimen
2A:
embryonic day 16.0
(more )
Specimen
2A:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 16.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: lung epithelium |
Present |
Regionally restricted |
2A
|
Low level of expression in epithelium of small airways. Foci of expression representing clusters of pulmonary neuroendocrine cells in epithelium of large airways.
|
Specimen
not shown:
embryonic day 17.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 17.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: lung epithelium |
Present |
Regionally restricted |
|
Pattern of expression similar to those described for more mature specimens of E16 lung tissue.
|
Specimen
3A:
embryonic day 18.0
(more )
Specimen
3A:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: lung epithelium |
Present |
Regionally restricted |
3A
|
Expression restricted to scatter foci occurring most frequently in large proximal airway epithelium, and more rarely in small airways.
|
Specimen
3C:
embryonic day 18.0
(more )
Specimen
3C:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: lung epithelium |
Present |
Regionally restricted |
3C
|
Expression in clusters of cells with the characteristic appearance of neuroepithelial bodies (NEBs). Expressing cells at the core of the NEB, with no expression in surrounding cells.
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Specimen
4A:
embryonic day 18.0
(more )
Specimen
4A:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: lung epithelium |
Present |
Regionally restricted |
4A
|
Expression in large NEB in large airway epithelium, and very small NEB in small airway.
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