Cert1<tm1a(KOMP)Wtsi> Targeted Allele Detail MGI Mouse (MGI:5287775)

Cert1^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

Symbol:	Cert1^{tm1a(KOMP)Wtsi}
Name:	ceramide transporter 1; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID:	MGI:5287775
Gene:	Cert1 Location: Chr13:96679243-96776675 bp, + strand Genetic Position: Chr13, 50.58 cM
Alliance:	Cert1^{tm1a(KOMP)Wtsi} page
IMPC:	Cert1 gene page

Mutation
origin

Mutant Cell Lines:	EPD0855_2_A11, EPD0855_2_A12, EPD0855_2_B11, EPD0855_2_C10, EPD0855_2_C12, EPD0855_2_D09, EPD0855_2_D10, EPD0855_2_D11, EPD0855_2_E10, EPD0855_2_E11, EPD0855_2_F10, EPD0855_2_H11
Germline Transmission:	Earliest citation of germline transmission: J:175295
Parent Cell Line:	JM8A3.N1 (ES Cell)
Strain of Origin:	C57BL/6N-A^tm1Brd
Project Collection:	KOMP-CSD

Mutation
description

Allele Type:		Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:		Insertion Vector: L1L2_Bact_P
		Mutation details: The L1L2_Bact_P cassette was inserted at position 96735477 of Chromosome 13 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 96736293. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 1 line available
Carrying any Cert1 Mutation:	28 strains or lines available

References

Original:	J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:	9 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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