Gtf2h5 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:174767 Tang F, et al., Deterministic and stochastic allele specific gene expression in single mouse blastomeres. PLoS One. 2011;6(6):e21208
Assay type:	RT-PCR
MGI Accession ID:	MGI:5289269
Gene symbol:	Gtf2h5
Gene name:	general transcription factor IIH, polypeptide 5

Probe:

Gtf2h5-assay_13/18-FP_first allele, RP

Assay notes:

These results were reported in Table S7. They were obtained using TaqMan real time PCR using individual blastomeres from 4-cell embryos. Two different primer sets were used with each sample in order to examine allele specific expression. The assay id presented in the raw CT values sheet of Table S7 differed from that associated with the primers in the assays design sheet; however, since results from only one assay were presented for this gene, it was assumed both ids referred to the same data. In the Supplementary Materials and Methods, the authors reported that a gene was detected when the real-time PCR reaction had a Ct value of <32. Thus, we have interpreted Ct values of <=31.5 as present and >=32.5 as absent.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
4-cell-E1-B1-first allele	E2.0	TS3: 4-cell stage embryo	Present (a)	Not Specified; total RNA	MF1		Not Specified	This reaction used blastomere 1 from embryo 1.
4-cell-E1-B1-second allele	Lane not used in this assay
4-cell-E1-B2-first allele	E2.0	TS3: 4-cell stage embryo	Present (b)	Not Specified; total RNA	MF1		Not Specified	This reaction used blastomere 2 from embryo 1.
4-cell-E1-B2-second allele	Lane not used in this assay
4-cell-E2-B1-first allele	E2.0	TS3: 4-cell stage embryo	Present (c)	Not Specified; total RNA	MF1		Not Specified	This reaction used blastomere 1 from embryo 2.
4-cell-E2-B1-second allele	Lane not used in this assay
4-cell-E2-B2-first allele	E2.0	TS3: 4-cell stage embryo	Present (d)	Not Specified; total RNA	MF1		Not Specified	This reaction used blastomere 2 from embryo 2.
4-cell-E2-B2-second allele	Lane not used in this assay
4-cell-E3-B1-first allele	E2.0	TS3: 4-cell stage embryo	Present (e)	Not Specified; total RNA	MF1		Not Specified	This reaction used blastomere 1 from embryo 3.
4-cell-E3-B1-second allele	Lane not used in this assay
4-cell-E3-B2-first allele	E2.0	TS3: 4-cell stage embryo	Present (f)	Not Specified; total RNA	MF1		Not Specified	This reaction used blastomere 2 from embryo 3.
4-cell-E3-B2-second allele	Lane not used in this assay

Notes:
(a) The averaged Ct value from 3 technical replicates was 28.66802333.
(b) The averaged Ct value from 3 technical replicates was 28.756171.
(c) The averaged Ct value from 3 technical replicates was 21.04471167.
(d) The averaged Ct value from 3 technical replicates was 20.33860033.
(e) The averaged Ct value from 3 technical replicates was 21.43935533.
(f) The averaged Ct value from 3 technical replicates was 28.02074667.

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