Probe:	Tcf-1 cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS21: thymus primordium	Strong		2A
TS21: tooth	Present	Regionally restricted	2A	Expression is present in odontoblasts.
TS21: basioccipital pre-cartilage condensation	Present		2A
TS21: palatal shelf	Present		2A	Expression is present in the palate.
TS21: spinal cord meninges	Present		2A	Expression is present in meninges surrounding the neural canal.
TS21: choroid plexus	Weak		2A
TS21: lung mesenchyme	Present		2A
TS21: metanephros	Present		2A
TS21: paramesonephric duct	Present		2A
TS21: urogenital sinus	Present		2A
TS21: oral region gland	Present	Regionally restricted	2A	Expression is present in the cortex of the adrenal gland. It is not present in the medulla.
TS21: genital tubercle	Present	Regionally restricted	2A	Expression is present in the anterior and posterior sites of the genital eminence.
TS21: hindlimb digit	Present	Regionally restricted	2A	Expression is present at the apex.
TS21: rib cartilage condensation	Present		2A	Expression is present around the thoracic ribs.
TS21: lower lip	Weak		2A
TS21: upper lip	Weak		2A
TS21: thoracic vertebral cartilage condensation	Present		2A	Expression is present in thoracic prevertebrae.
TS21: tail vertebral cartilage condensation	Absent		2A	There is no expression in tail prevertebrae.
TS21: saccule mesenchyme	Absent		2A	There is no expression present in the mesenchymal cells of the inner ear around the cochlea.

Structure	Level	Pattern	Image	Note
TS22: embryo	Present	Non-Uniform	2B	Expression is similar to that seen at E13.5.
TS22: viscerocranium	Present	Regionally restricted	2B	Expression is present in the cartilage primordium of the nasal bone.
TS22: mandible	Present		2B
TS22: maxilla	Present		2B
TS22: palatal shelf	Present		2B
TS22: lower lip	Absent		2B
TS22: upper lip	Absent		2B
TS22: rib cartilage condensation	Present		2B	Expression is present around the thoracic ribs.
TS22: thoracic vertebral cartilage condensation	Present		2B	Expression is present in thoracic prevertebrae.
TS22: mesenchyme of metanephros	Strong		2B	Expression is present in the mesenchymal cells surrounding the metanephros.
TS22: mesenchyme of urogenital sinus	Present		2B
TS22: paramesonephric duct of male	Present	Regionally restricted	2B	Expression is present in the mesenchymal cells.
TS22: adrenal cortex	Present		2B
TS22: adrenal medulla	Absent		2B
TS22: tail vertebral cartilage condensation	Absent		2B	There is no expression in tail prevertebrae.
TS22: hip	Absent		2B	There is no expression in the osteogenic cells.
TS22: pituitary gland	Absent		2B
TS22: midbrain	Absent		2B	There is no expression in the roof.
TS22: thalamus	Absent		2B	There is no expression in the dorsal thalamus.
TS22: hypothalamus	Absent		2B
TS22: saccule mesenchyme	Absent		2B	There is no expression in the mesenchymal cells of the inner ear around the cochlea.

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present		3A	Expression levels are higher in the mesoderm than the ectoderm. It is present in an anterioposterior gradient, with the highest levels being expressed posteriorly.
TS11: embryo mesoderm	Present		3A	Expression levels are higher in the mesoderm than the ectoderm. It is present in an anterioposterior gradient, with the highest levels being expressed posteriorly.
TS11: Reichert's membrane	Present		3A
TS11: amnion	Present		3A
TS11: chorion	Present		3A
TS11: trophectoderm	Present		3A	Expression is present in trophectoderm-derived cells.
TS11: embryo endoderm	Absent		3A

Structure	Level	Pattern	Image	Note
TS11: allantois	Present

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present		4A
TS15: trunk mesenchyme	Present	Regionally restricted	4A	Expression is present in part of the lateral plate mesoderm.
TS15: 1st branchial arch	Present		4A	It is likely expression occurs in neural crest-derived cells.
TS15: trunk mesenchyme derived from neural crest	Present		4A	Expression is present in mesenchymal cells that most likely derive from neural crest.

Structure	Level	Pattern	Image	Note
TS15: 2nd branchial arch	Present			It is likely expression occurs in neural crest-derived cells.

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Present	Regionally restricted	5A	Expression is present in the progress zone, between the apical ectodermal ridge and the marginal vein.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Regionally restricted	5B	Expression is present in cells clustered on either side of the neural tube, suggesting a neural crest origin.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present		5C	It is likely expression occurs in neural crest-derived cells.
TS17: 2nd branchial arch	Present		5C	It is likely expression occurs in neural crest-derived cells.
TS17: 3rd branchial arch	Present		5C	It is likely expression occurs in neural crest-derived cells.
TS17: 1st branchial arch ectoderm	Present		5C	Expression is present in the epithelial lining between the mandibular and maxillary component.
TS17: 3rd branchial arch ectoderm	Present		5C	Expression is present in the epithelium.

Structure	Level	Pattern	Image	Note
TS17: lung mesenchyme	Present		6A	Expression is present in the mesenchymal cells around the bronchioles.

Structure	Level	Pattern	Image	Note
TS21: lung mesenchyme	Present		6B	Expression is present in the mesenchymal cells around the bronchioles.
TS21: lung epithelium	Absent		6B	There is no expression in bronchiolar epithelial cells.
TS21: rib cartilage condensation	Present		6B	Expression is present around the thoracic ribs.

Structure	Level	Pattern	Image	Note
TS22: lung mesenchyme	Present			Expression is present in the mesenchymal cells around the bronchioles.
TS22: lung epithelium	Absent			There is no expression in bronchiolar epithelial cells.

Structure	Level	Pattern	Image	Note
TS17: lateral ventricle	Weak	Regionally restricted	7A	Expression is present in the developing choroid plexus.
TS17: 1st branchial arch mandibular component	Present		7A
TS17: nasal process	Present	Regionally restricted	7A	Expression is present in mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS17: mesonephros mesenchyme	Weak			Expression is present in mesenchymal cells surrounding the mesonephric tubules.

Structure	Level	Pattern	Image	Note
TS19: latero-nasal process mesenchyme	Present
TS19: medial-nasal process mesenchyme	Present
TS19: diencephalon	Absent
TS19: midbrain	Absent

Structure	Level	Pattern	Image	Note
TS27: lung	Absent
TS27: renal medullary interstitium	Trace			Minimal amounts of expression are occasionally detected in medullary mesenchymal cells.
TS27: hemolymphoid system	Present			Expression is present in lymphoid tissue.