Hprt1tm212(Ple168-EGFP/cre)Ems
Targeted Allele Detail
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| Symbol: |
Hprt1tm212(Ple168-EGFP/cre)Ems |
| Name: |
hypoxanthine phosphoribosyltransferase 1; targeted mutation 212, Elizabeth M Simpson |
| MGI ID: |
MGI:5303299 |
| Gene: |
Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
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| Mutant Cell Lines: |
mEMS948, mEMS949, mEMS1007, mEMS1009 |
| Germline Transmission: |
Unknown
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| Parent Cell Line: |
E14TG2a (ES Cell)
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| Strain of Origin: |
129P2/OlaHsd
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| Project Collection: |
Pleiades Promoter Project
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| Allele Type: |
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Targeted (Recombinase, Reporter) |
| Mutation: |
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Insertion
Vector Type: Not Applicable
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Hprt1tm212(Ple168-EGFP/cre)Ems expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Homolog in Mouse |
Note |
| human |
POGZ (23126) |
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Mutation details: The Ple168-EGFP/Cre plasmid (pEMS1092) was created by inserting the Ple168 MiniPromoter (derived from specific promoter/enhancer/regulatory regions of the human pogo transposable element with ZNF domain (POGZ) gene) into the multiple cloning site of the pEMS1302 plasmid vector backbone. This placed the Ple168 MiniPromoter upstream of a minimal F5 mutant-frt site, a Kozak consensus sequence, an EGFP/Cre fusion gene (enhanced green fluorescent protein (EGFP; with mutated TAA to TTA stop) and Cre recombinase gene), a nuclear localization signal, a minimal wildtype frt site, an SV40 early polyA signal, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. This Ple168-EGFP/Cre construct was targeted as a single copy knock-in to the Hprtb-m3 mutant locus on the X Chromosome via electroporation into E14TG2a embryonic stem cells.
(J:164356)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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POGZ
(human)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Hprt1 Mutation: |
1279 strains or lines available
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| Original: |
J:164356 Portales-Casamar E, et al., A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16589-94 |
| All: |
1 reference(s) |
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Analysis Tools
