Hprt1tm325(Ple157-cre/EGFP)Ems
Targeted Allele Detail
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| Symbol: |
Hprt1tm325(Ple157-cre/EGFP)Ems |
| Name: |
hypoxanthine phosphoribosyltransferase 1; targeted mutation 325, Elizabeth M Simpson |
| MGI ID: |
MGI:5303373 |
| Gene: |
Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
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| Mutant Cell Line: |
mEMS569 |
| Germline Transmission: |
Unknown
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| Parent Cell Line: |
E14TG2a (ES Cell)
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| Strain of Origin: |
129P2/OlaHsd
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| Project Collection: |
Pleiades Promoter Project
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| Allele Type: |
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Targeted (Recombinase, Reporter) |
| Mutation: |
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Insertion
Vector Type: Not Applicable
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Hprt1tm325(Ple157-cre/EGFP)Ems expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Homolog in Mouse |
Note |
| human |
PCP2 (126006) |
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Mutation details: The Ple157-Cre/EGFP plasmid (pEMS1064) was created by inserting the Ple157 MiniPromoter (derived from specific promoter/enhancer/regulatory regions of the human Purkinje cell protein 2 (PCP2) gene) into the multiple cloning site of the pEMS1040 plasmid vector backbone. This placed the Ple157 MiniPromoter upstream of a minimal wildtype frt site, a Cre/EGFP fusion gene (Cre recombinase gene and enhanced green fluorescent protein (EGFP; with mutated TAA->TTA stop)), a nuclear localization signal, an SV40 early mRNA polyA signal, a modified minimal frt site (A deletion), a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. This Ple157-Cre/EGFP construct was targeted as a single copy knock-in to the Hprtb-m3 mutant locus on the X Chromosome via electroporation into E14TG2a embryonic stem cells: a male ES cell line harboring the Hprtb-m3 mutation on the X Chromosome. The resulting ES cells, correctly targeted with the Ple157-Cre/EGFP transgene inserted into the Hprt locus, were assigned Hprttm28(Ple157-cre/EGFP)Ems.
(J:164356)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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PCP2
(human)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Hprt1 Mutation: |
1279 strains or lines available
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| Original: |
J:164356 Portales-Casamar E, et al., A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16589-94 |
| All: |
1 reference(s) |
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Analysis Tools
