Hprt1tm294(Ple232-EGFP/cre)Ems
Targeted Allele Detail
|
|
| Symbol: |
Hprt1tm294(Ple232-EGFP/cre)Ems |
| Name: |
hypoxanthine phosphoribosyltransferase 1; targeted mutation 294, Elizabeth M Simpson |
| MGI ID: |
MGI:5303391 |
| Gene: |
Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
|
|
| Mutant Cell Lines: |
mEMS882, mEMS884, mEMS885, mEMS907 |
| Germline Transmission: |
Unknown
|
| Parent Cell Line: |
E14TG2a (ES Cell)
|
| Strain of Origin: |
129P2/OlaHsd
|
| Project Collection: |
Pleiades Promoter Project
|
|
| Allele Type: |
|
Targeted (Recombinase, Reporter) |
| Mutation: |
|
Insertion
Vector Type: Not Applicable
|
| |
|
Hprt1tm294(Ple232-EGFP/cre)Ems expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Homolog in Mouse |
Note |
| human |
TNNT1 (7138) |
|
|
|
| |
|
Mutation details: The Ple232-EGFP/Cre plasmid (pEMS1100) was created by inserting the Ple232 MiniPromoter (derived from specific promoter/enhancer/regulatory regions of the human troponin T type 1 (skeletal, slow) (TNNT1) gene) into the multiple cloning site of the pEMS1302 plasmid vector backbone. This placed the Ple232 MiniPromoter upstream of a minimal F5 mutant-frt site, a Kozak consensus sequence, an EGFP/Cre fusion gene (enhanced green fluorescent protein (EGFP; with mutated TAA to TTA stop) and Cre recombinase gene), a nuclear localization signal, a minimal wildtype frt site, an SV40 early polyA signal, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. This Ple232-EGFP/Cre construct was targeted as a single copy knock-in to the Hprtb-m3 mutant locus on the X Chromosome via electroporation into E14TG2a embryonic stem cells.
(J:164356)
|
|
|
|
Activity: |
 Tissue activity of this recombinase allele
|
|
Driver:
|
TNNT1
(human)
|
| |
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
| Carrying any Hprt1 Mutation: |
1279 strains or lines available
|
|
| Original: |
J:164356 Portales-Casamar E, et al., A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16589-94 |
| All: |
1 reference(s) |
|
Analysis Tools
