Hprt1tm328(EGFP/cre)Ems
Targeted Allele Detail
|
|
| Symbol: |
Hprt1tm328(EGFP/cre)Ems |
| Name: |
hypoxanthine phosphoribosyltransferase 1; targeted mutation 328, Elizabeth M Simpson |
| MGI ID: |
MGI:5303418 |
| Gene: |
Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
|
|
|
|
| Allele Type: |
|
Targeted (Recombinase, Reporter) |
| Mutation: |
|
Insertion
Vector Type: Not Applicable
|
| |
|
Hprt1tm328(EGFP/cre)Ems expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Hprt1 (MGI:96217) |
|
|
| |
|
Mutation details: The promoterless-EGFP/Cre plasmid (pEMS1308) was created by inserting no Pleiades Promoter into the multiple cloning site of the pEMS1308 plasmid vector backbone. Therefore, no promoter is upstream of a minimal F5 mutant-frt site, a Kozak consensus sequence, an EGFP/Cre fusion gene (enhanced green fluorescent protein (EGFP; with mutated TAA->TTA stop) and Cre recombinase gene), a minimal wildtype frt site, an SV40 early polyA signal, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. This promoterless-EGFP/Cre construct was targeted as a single copy knock-in to the Hprtb-m3 mutant locus on the X Chromosome via electroporation into mEMS1202 embryonic stem cells: a male ES cell line from a B6129F1-Gt(ROSA)26Sor+/+ Hprtb-m3/Y mouse (F1 generation male pups born of C57BL/6J-congenic females homozygous for a Hprtb-m3 mutation [originally derived from 129P2/OlaHsd-derived E14TG2a embryonic stem cells] X 129S-Gt(ROSA)26Sortm1Sor heterozygous males (see Stock No. 002171 and 003310, respectively)). The resulting ES cells, correctly targeted with the promoterless-EGFP/Cre transgene inserted into the Hprt locus, were assigned Hprttm318(EGFP/cre)Ems.
(J:164356)
|
|
|
|
Activity: |
 Tissue activity of this recombinase allele
|
|
Driver:
|
Hprt1
(mouse)
|
| |
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
| Carrying any Hprt1 Mutation: |
1279 strains or lines available
|
|
| Original: |
J:164356 Portales-Casamar E, et al., A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16589-94 |
| All: |
1 reference(s) |
|
Analysis Tools
