Col1a1<tm1(tetO-RNAi:Rps19)Karl> Targeted Allele Detail MGI Mouse (MGI:5304754)

Col1a1^{tm1(tetO-RNAi:Rps19)Karl}
Targeted Allele Detail

Summary

Symbol:	Col1a1^{tm1(tetO-RNAi:Rps19)Karl}
Name:	collagen, type I, alpha 1; targeted mutation 1, Stefan Karlsson
MGI ID:	MGI:5304754
Synonyms:	shRNA-B
Gene:	Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance:	Col1a1^{tm1(tetO-RNAi:Rps19)Karl} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:179085
Parent Cell Line:	KH2 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Mutation
description

Allele Type:		Targeted (Inducible, Knockdown)
Inducer:		doxycycline/tetracycline
Mutation:		Insertion
		Col1a1^{tm1(tetO-RNAi:Rps19)Karl} involves 1 genes/genome features (Rps19) View all
		Mutation details: RMCE on KH2 cells inserts an shRNA targeting Rsp19 behind a tetracycline responsive element and CMV promoter replacing the Pou5f1 cassette in Col1a1^{tm2(tetO-Pou5f1)Jae} into the 3'UTR. The level of knock down by this allele following doxycycline treatment is lower compared to mice expressing Col1a1^{tm2(tetO-RNAi:Rps19)Karl}. (J:179085)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Disease models

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:	163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae}. This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

Original:	J:179085 Jaako P, et al., Mice with ribosomal protein S19 deficiency develop bone marrow failure and symptoms like patients with Diamond-Blackfan anemia. Blood. 2011 Dec 1;118(23):6087-96
All:	3 reference(s)

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