Notch4<tm1.1(cre/ERT2)Sat> Targeted Allele Detail MGI Mouse (MGI:5304916)

Notch4^{tm1.1(cre/ERT2)Sat}
Targeted Allele Detail

Summary

Symbol:	Notch4^{tm1.1(cre/ERT2)Sat}
Name:	notch 4; targeted mutation 1.1, Spyros Artavanis-Tsakonas
MGI ID:	MGI:5304916
Synonyms:	N4-CreERT2^SAT
Gene:	Notch4 Location: Chr17:34783242-34807477 bp, + strand Genetic Position: Chr17, 18.15 cM
Alliance:	Notch4^{tm1.1(cre/ERT2)Sat} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:179632
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	C57BL/6NTac

Mutation
description

Allele Type:

Targeted (Inducible, Recombinase)

Inducer:

tamoxifen

Mutations:

Insertion, Intragenic deletion

Notch4^{tm1.1(cre/ERT2)Sat} expression driven by 1 gene

Mutation details: A targeting vector was constructed to insert the cre/ERT2 coding sequence, a polyadenylation signal and an Frt-flanked neo selection cassette in-frame with the start codon of Notch 4, by homologous recombination in ES cells. This resulted in deletion of most of exon 1. Chimeric males were bred to Flpe-expressing females to remove the selection cassette. (J:179632)

Generation of the various Notch knock-in mice

Recombinase
activity

Activity:	Tissue activity of this recombinase allele
Driver:	Notch4 (mouse) Summary of all recombinase alleles driven by Notch4. Alliance mouse Notch4 gene page

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available Cell Lines: 0 lines available
Carrying any Notch4 Mutation:	87 strains or lines available

References

Original:	J:179632 Fre S, et al., Notch lineages and activity in intestinal stem cells determined by a new set of knock-in mice. PLoS One. 2011;6(10):e25785
All:	1 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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