Col1a1<tm9(tetO-Dnmt3b_i1)Jae> Targeted Allele Detail MGI Mouse (MGI:5313405)

Col1a1^{tm9(tetO-Dnmt3b_i1)Jae}
Targeted Allele Detail

Summary

Symbol:	Col1a1^{tm9(tetO-Dnmt3b_i1)Jae}
Name:	collagen, type I, alpha 1; targeted mutation 9, Rudolf Jaenisch
MGI ID:	MGI:5313405
Synonyms:	tetO-Dnmt3b1
Gene:	Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance:	Col1a1^{tm9(tetO-Dnmt3b_i1)Jae} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:127808
Parent Cell Line:	KH2 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Mutation
description

Allele Type:

Targeted (Inducible, Inserted expressed sequence, RMCE-ready)

Inducer:

doxycycline/tetracycline

Mutation:

Insertion

Col1a1^{tm9(tetO-Dnmt3b_i1)Jae} expresses 1 gene

Mutation details: RMCE on KH2 cells inserted a cassette that contains a splice acceptor-double polyA sequence and the tetracycline responsive element (tetO) upstream of the DNA methyltransferase 3B gene isoform 1 (Dnmt3b) into the 3'UTR. (J:127808)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:	163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae} (J:159351). This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

Original:	J:127808 Linhart HG, et al., Dnmt3b promotes tumorigenesis in vivo by gene-specific de novo methylation and transcriptional silencing. Genes Dev. 2007 Dec 1;21(23):3110-22
All:	4 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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