Tg(Tbx6-cre/ERT2)5769Fan
Transgene Detail
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| Symbol: |
Tg(Tbx6-cre/ERT2)5769Fan |
| Name: |
transgene insertion 5769, Chen-Ming Fan |
| MGI ID: |
MGI:5431548 |
| Synonyms: |
Tbx6;CreERT2, Tbx6;CreERTg5769 |
| Transgene: |
Tg(Tbx6-cre/ERT2)5769Fan Location: unknown
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| Alliance: |
Tg(Tbx6-cre/ERT2)5769Fan page
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| Transgene Type: |
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Transgenic (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Tg(Tbx6-cre/ERT2)5769Fan expression driven by
1 gene
Transgene expression driven by:
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Mutation details: A region (containing the Tbx6 enhancer and promoter fragment) -2.5 kb upstream and +25 bp downstream of the Tbx6 transcriptional start site was amplified from a BAC template (RP24-239G12). This fragment was cloned 5' to a cre/ERT2 expression cassette in a plasmid. The linearized, purified transgene was injected into pronuclei of (C57BL/6 x DBA/2)F1 embryos. 16 founders cre-positive founders were identified by PCR. Each founder was bred to ROSA-lacz reporter mice and pregnant mice were treated with tamoxifen. 6 lines were sterile, lacked cre activity or showed ubiquitous activity in all mesodermal tissues. 4 lines showed mosaic activity in the presomitic mesoderm (represented by line 5578). The remaining 6 lines had tightly-regulated tamoxifen-inducible cre activity, with line 5679 being further characterized due to tamoxifen-regulated PSM activity.
(J:185483)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Tbx6
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:185483 Peter Lopez T, et al., A transgenic Tbx6;CreER(T2) line for inducible gene manipulation in the presomitic mesoderm. Genesis. 2012 Jun;50(6):490-5 |
| All: |
1 reference(s) |
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Analysis Tools
