Tg(Nes-phiC31*)1Imayo
Transgene Detail
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| Symbol: |
Tg(Nes-phiC31*)1Imayo |
| Name: |
transgene insertion 1, Itaru Imayoshi |
| MGI ID: |
MGI:5431596 |
| Synonyms: |
Nestin-PhiC31o |
| Transgene: |
Tg(Nes-phiC31*)1Imayo Location: unknown
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| Alliance: |
Tg(Nes-phiC31*)1Imayo page
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| Strain of Origin: |
C57BL/6J or (C57BL/6 x DBA)F1
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| Transgene Type: |
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Transgenic (Recombinase) |
| Mutation: |
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Insertion
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Tg(Nes-phiC31*)1Imayo expression driven by
1 gene
Transgene expression driven by:
| Organism |
Driver Gene |
Note |
| Not Specified |
Nes |
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Mutation details: The construct was composed of a 5.8 kb fragment of the nestin promoter upstream of the sequence for optimized phiC31 recombinase (phiC31*), with the 1.8 kb second intron containing the neural stem/progenitor cell-specific enhancer downstream of the recombinase sequence. The SV40 polyadenylation sequence was also cloned into the vector. The linearized, purified construct was injected into pronuclei of fertilized one-cell embyros. Only one founder line was established. Mice show wide expression of phiC31 mRNA in the nervous system with no expression observed in non-neural tissues like somites. High expression is observed in the ventricular zone of the developing nervous system.
(J:185547)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Nes
(species not specified)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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| Original: |
J:185547 Imayoshi I, et al., In vivo evaluation of PhiC31 recombinase activity in transgenic mice. Neurosci Res. 2012 Jun;73(2):106-14 |
| All: |
1 reference(s) |
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Analysis Tools
