Tg(Nphs1-flpo)1Jhm
Transgene Detail
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| Symbol: |
Tg(Nphs1-flpo)1Jhm |
| Name: |
transgene insertion 1, Jeffrey H Miner |
| MGI ID: |
MGI:5431870 |
| Synonyms: |
Nphs1-FLPo |
| Transgene: |
Tg(Nphs1-flpo)1Jhm Location: unknown
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| Alliance: |
Tg(Nphs1-flpo)1Jhm page
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| Strain of Origin: |
(C57BL/6J x CBA/J)F2
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| Transgene Type: |
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Transgenic (Recombinase) |
| Mutation: |
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Insertion
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Tg(Nphs1-flpo)1Jhm expression driven by
1 gene
Transgene expression driven by:
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Mutation details: A 4.2 kb fragment of the mouse Nphs1 (nephrin) promoter was cloned upstream of the optimized Flp recombinase (Flpo) with a 3' bovine growth hormone polyadenylation sequence. The construct was microinjected into fertilized pronuclei. 18 founders were obtained with 3 demonstrating excision of the Frt-flanked neo from the Lama5neo allele. Flpo mRNA was detected by in situ hybridization in all three lines (1,3,5). Lines 1 and 3 when crossed to Lama5tm3Jhm mice showed higher podocyte expression of FLPo and normal tubular basement membrane levels of laminin alpha-5 (Lama5).
(J:185850)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Nphs1
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:185850 Goldberg S, et al., Maintenance of glomerular filtration barrier integrity requires laminin alpha5. J Am Soc Nephrol. 2010 Apr;21(4):579-86 |
| All: |
2 reference(s) |
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Analysis Tools