Probe:	IMAGE clone 4188081
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	2B	Expression was detected in the ventral diencephalon, encompassing the entire prospective infundibulum and extending into the posterior hypothalamus.
TS15: oral region ectoderm	Present	Regionally restricted	2B	Expression was detected in the invaginating oral ectoderm.
TS15: Rathke's pouch	Present		2B

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	3D	Expression was detected in the ventral diencephalon.
TS19: infundibular recess of 3rd ventricle	Present		3D
TS19: Rathke's pouch	Present	Regionally restricted	3D	Expression was detected in the rostral tip of Rathke's pouch. At E11.5 expression partially overlaps with Lhx3 and Isl1.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted	3E	Expression was detected in the ventral diencephalon.
TS21: remnant of Rathke's pouch	Present	Regionally restricted	3E	Expression was confined to a specific cell population at the rostral tip of the gland. Expression was mutually exclusive with Lhx3.

Structure	Level	Pattern	Image	Note
TS23: diencephalon	Present	Regionally restricted	3F	Expression was detected in the ventral diencephalon.
TS23: adenohypophysis	Absent		3F
TS23: neurohypophysis	Present		3F

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	4F	Expression was detected in ventral diencephalon close to the hypothalamic region.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	4G	Expression was detected in ventral diencephalon.
TS19: Rathke's pouch	Present		4G

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	4H	Expression was detected in ventral diencephalon.
TS19: Rathke's pouch	Present		4H

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	4I	Expression was detected in ventral diencephalon.
TS19: infundibular recess of 3rd ventricle	Present		4I

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	4J	Expression was detected in ventral diencephalon.
TS19: infundibular recess of 3rd ventricle	Present		4J

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	7B	Expression was detected in migrating branchiomotor/visceromotor neurons.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	7G	Expression was detected in migrating branchiomotor/visceromotor neurons.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	7L	Expression was detected in migrating branchiomotor/visceromotor neurons.

Structure	Level	Pattern	Image	Note
TS19: facial ganglion	Present		7E'

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Present		7I'	Expression was detected in the mandibular process.

Structure	Level	Pattern	Image	Note
TS21: tongue epithelium	Present	Regionally restricted	7J'	Expression was detected in the tongue neuroepithelium.

Structure	Level	Pattern	Image	Note
TS23: tongue epithelium	Present	Regionally restricted	7K'	Expression was detected in the tongue neuroepithelium.