Tg(CAG-flpe/ERT2)1Dym
Transgene Detail
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| Symbol: |
Tg(CAG-flpe/ERT2)1Dym |
| Name: |
transgene insertion 1, Susan Dymecki |
| MGI ID: |
MGI:5432116 |
| Synonyms: |
CAG::FlpeERT2 |
| Transgene: |
Tg(CAG-flpe/ERT2)1Dym Location: unknown
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| Alliance: |
Tg(CAG-flpe/ERT2)1Dym page
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| Strain of Origin: |
(C57BL/6 x SJL)F2 or FVB
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| Transgene Type: |
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Transgenic (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Tg(CAG-flpe/ERT2)1Dym expression driven by
1 gene
Transgene expression driven by:
| Organism |
Driver Gene |
Note |
| chicken |
CAG |
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Mutation details: The construct was constructed by inserting a cassette containing the FLPe/ERT2 coding and polyA sequences (enhanced FLP fused to a triple mutant estrogen receptor) into a vector containing the CAG promoter (chicken beta-actin promoter coupled to CMV early enhancer sequence). Tandem insulator sequences that include the chicken beta globin hypersensitive sites flanked the entire construct. The purified transgene was injected into fertilized eggs to produce transgenic animals. Eight lines were obtained. After treatment with tamoxifen, all showed ability to recombine and activate the R26::FRAP reporter when crossed to mice carrying that allele. The line showing the greatest induction of alkaline phophatase activity in embryos was characterized in further detail. Cre activity was found to be low in most adult tissues and not effective in the spleen or skin (J:186310).
(J:97320)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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CAG
(chicken)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:97320 Hunter NL, et al., Ligand-activated Flpe for temporally regulated gene modifications. Genesis. 2005 Mar;41(3):99-109 |
| All: |
3 reference(s) |
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Analysis Tools
