Summary | Mutation origin | Mutation description | Recombinase activity | Phenotypes | Tumor data | Find Mice (IMSR) | Notes | References

Symbol:	Gt(ROSA)26Sortm3(CAG-flpo/ERT2)Alj
Name:	gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Alexandra L Joyner
MGI ID:	MGI:5432216
Synonyms:	Gt(ROSA)26Sortm3(ACTB-FLPo/ERT2)Alj, R26FlpoER
Gene:	Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance:	Gt(ROSA)26Sortm3(CAG-flpo/ERT2)Alj page

Germline Transmission:	Earliest citation of germline transmission: J:186310
Parent Cell Line:	Other (see notes) (ES Cell)
Strain of Origin:	129S6/SvEvTac

Allele Type:    Targeted (Inducible, Recombinase) Inducer:    tamoxifen Mutation:    Insertion   Gt(ROSA)26Sortm3(CAG-flpo/ERT2)Alj expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note chicken CAG   Mutation details: The sequence encoding a FLPo/ERT2 fusion protein (optimized FLP recombinase fused to the tamoxifen-responsive mutated human estrogen receptor ligand-binding domain), SV40 polyadenylation site and a loxP-flanked PGK-neo cassette was cloned downstream of the human CMV enhancer/chicken ACTB promoter (CAG). This construct was targeted to the Gt(ROSA)26Sor locus in embryonic stem cells. (J:186310)

Activity:	Tissue activity of this recombinase allele
Driver:	CAG (chicken) Summary of all recombinase alleles driven by CAG.

View phenotypes and curated references for all genotypes (concatenated display).

List all tumor models in MMHCdb carrying Gt(ROSA)26Sortm3(CAG-flpo/ERT2)Alj

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:	993 strains or lines available

Original:	J:186310 Lao Z, et al., MASTR: a technique for mosaic mutant analysis with spatial and temporal control of recombination using conditional floxed alleles in mice. Cell Rep. 2012 Aug 30;2(2):386-96
All:	15 reference(s)