Probe:	Irx3 cDNA6
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS11: extraembryonic ectoderm	Present		3A

Structure	Level	Pattern	Image	Note
TS11: chorion ectoderm	Present		3A'
TS11: embryo ectoderm	Present		3A'
TS11: extraembryonic ectoderm	Present		3A'

Structure	Level	Pattern	Image	Note
TS12: future hindbrain neural fold	Strong		3B
TS12: future midbrain neural fold	Strong		3B

Structure	Level	Pattern	Image	Note
TS12: future hindbrain neural fold	Strong		3B'
TS12: future prosencephalon	Absent		3B'

Structure	Level	Pattern	Image	Note
TS13: future spinal cord	Present	Regionally restricted	3C	Expression is present in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present		3C'
TS13: future midbrain	Present		3C'
TS13: future spinal cord	Present	Regionally restricted	3C'	Expression is present in the rostral part of the spinal cord.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	3C''	Expression is present in the ventricular zone, excluding the floor and roof plates.
TS13: future hindbrain floor plate	Absent		3C''
TS13: head mesenchyme	Present		3C''	Expression is present in cephalic mesodermal cells.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Strong		3D
TS15: future midbrain	Strong		3D
TS15: future spinal cord	Strong		3D
TS15: 1st branchial arch ectoderm	Strong		3D	Authors reported strong expression in the ectodermal layer of the branchial arches.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Strong		3D'
TS15: head mesenchyme	Present		3D'	Expression is present in the cephalic mesoderm.
TS15: trunk mesenchyme	Present	Regionally restricted	3D'	Expression is present in the lateral plate mesoderm.