Tg(Dcx-cre/ERT2)DC-F18Zqx
Transgene Detail
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| Symbol: |
Tg(Dcx-cre/ERT2)DC-F18Zqx |
| Name: |
transgene insertion DC-F18, Zhi-Qi Xiong |
| MGI ID: |
MGI:5438982 |
| Synonyms: |
DC-F18, Dcx-CreERT2 |
| Transgene: |
Tg(Dcx-cre/ERT2)DC-F18Zqx Location: unknown
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| Alliance: |
Tg(Dcx-cre/ERT2)DC-F18Zqx page
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| Transgene Type: |
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Transgenic (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Tg(Dcx-cre/ERT2)DC-F18Zqx expression driven by
1 gene
Transgene expression driven by:
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Mutation details: A BAC clone (RP23-462G16) containing the Dcx locus as well as 30 kb upstream and 60 kb downstream was homologously recombined with a targeting vector to insert the cre/ERT2 open reading frame, a bovine growth hormone polyA tag and an Frt-flanked neo marker into exon 2 of Dcx which silenced the BAC copy of Dcx. Arabinose-induced flp expression in bacterial cells removed the neo cassette from the construct. The linearized, purified construct was microinjected into pronuclei of fertilized C57BL/6 eggs. Founders were crossed to cre-responsive Rosa26-lacz mice to identify animals with the neural expression of cre. Line DC-F18 showed tamoxifen-induced cre activity in the subgranular zone (SGZ) of the dentate gyrus, but not in the subventricular zone (SVZ), with non-specific labeling in some neurons of the piriform cortex, amygdala, and hypothalamus also observed. This line was analyzed in further detail in the SGZ.
(J:187505)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Dcx
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:187505 Cheng X, et al., Pulse labeling and long-term tracing of newborn neurons in the adult subgranular zone. Cell Res. 2011 Feb;21(2):338-49 |
| All: |
9 reference(s) |
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Analysis Tools
