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Tcf21tm3.1(cre/Esr1*)Eno
Targeted Allele Detail
Summary
Symbol: Tcf21tm3.1(cre/Esr1*)Eno
Name: transcription factor 21; targeted mutation 3.1, Eric N Olson
MGI ID: MGI:5442346
Synonyms: Tcf21iCre, Tcf21MCM, Tcf21-MerCreMer
Gene: Tcf21  Location: Chr10:22693162-22696042 bp, - strand  Genetic Position: Chr10, 10.33 cM
Alliance: Tcf21tm3.1(cre/Esr1*)Eno page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:188837
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted (Inducible, Recombinase)
Inducer:    tamoxifen
Mutations:    Insertion, Intragenic deletion
 
Tcf21tm3.1(cre/Esr1*)Eno expression driven by 1 gene
 
Mutation details: A targeting vector was designed to replace exon 1 of the transcription factor 21,Tcf21 (capsulin; or epicardin) with a mercremer protein followed by a frt-flanked neo cassette. cre recombinase cDNA sequence flanked on each end with a mutated murine estrogen receptor (mer) ligand-binding domain. Flp-mediated recombination deletes the neo cassette. (J:188837)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Tcf21 (mouse)
Summary of all recombinase alleles driven by Tcf21.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Tcf21 Mutation:  16 strains or lines available
Notes
The construct was electroporated into 129SvEvTac embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6 mice to establish a colony.These mice were subsequently bred to Tg(ACTFLPe)9205Dym mice to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene.
References
Original:  J:188837 Song K, et al., Heart repair by reprogramming non-myocytes with cardiac transcription factors. Nature. 2012 May 31;485(7400):599-604
All:  33 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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11/19/2024
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