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Gene Expression Data
Assay Details
Assay
Reference: J:39657 Lobe CG, Expression of the helix-loop-helix factor, Hes3, during embryo development suggests a role in early midbrain-hindbrain patterning. Mech Dev. 1997 Mar;62(2):227-37
Assay type: RNA in situ
MGI Accession ID: MGI:5444151
Gene symbol: Ascl1
Gene name: achaete-scute family bHLH transcription factor 1
Probe: Mash1 cDNA9
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 4D: embryonic day 9.0 (more )
Structure Level Pattern Image Note
TS15: future midbrain Present Regionally restricted 4D Expressed in the dorsal part of the mesencephalon.
Specimen 4E: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: future midbrain Present Regionally restricted 4E Expressed in the dorsal part of the mesencephalon.
TS15: future hindbrain Present Regionally restricted 4E Expressed in the dorsal part of the hindbrain.
TS15: neural tube Present Regionally restricted 4E Expressed in the dorsal part.
TS15: midbrain-hindbrain junction Absent 4E No expression in the cerebello-mesencephalic flexure.
Specimen 4F: embryonic day 9.5 (more )
Note: A slightly older embryo than that shown in 4B.
Structure Level Pattern Image Note
TS15: future forebrain Present 4F Expression is stronger throughout the central nervous system, similar to Hes3 expression at the same stage
TS15: future hindbrain Present 4F Expression is stronger throughout the central nervous system, similar to Hes3 expression at the same stage
TS15: future midbrain Present 4F Expression is stronger throughout the central nervous system, similar to Hes3 expression at the same stage
TS15: neural tube Present 4F Expression is stronger throughout the central nervous system, similar to Hes3 expression at the same stage
Specimen 4I: embryonic day 9.5 (more )
Note: Cross-sections of the embryo shown in (F).
Structure Level Pattern Image Note
TS15: future hindbrain Present Regionally restricted 4I Expressed in the dorsal part of the myelencephalon, more dorsal than Hes3 expression region.
TS15: future hindbrain lateral wall Present Regionally restricted 4I Expressed in cells flanking the floor plate.
Specimen 5C: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS21: spinal cord ventricular layer Present Widespread 5C Expression is strongest throughout the dorsal ventricular zone.
TS21: spinal cord floor plate Absent 5C
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
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