Abcg2tm3.1(cre/ERT2)Bsor
Targeted Allele Detail
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| Symbol: |
Abcg2tm3.1(cre/ERT2)Bsor |
| Name: |
ATP binding cassette subfamily G member 2 (Junior blood group); targeted mutation 3.1, Brian P Sorrentino |
| MGI ID: |
MGI:5444690 |
| Synonyms: |
Abcg2CreERT2, Abcg2-Ires-CreERT2 |
| Gene: |
Abcg2 Location: Chr6:58561476-58669436 bp, + strand
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| Alliance: |
Abcg2tm3.1(cre/ERT2)Bsor page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:189206
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| Parent Cell Line: |
F12 (ES Cell)
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| Strain of Origin: |
129X1/SvJ
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| Allele Type: |
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Targeted (Hypomorph, Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Abcg2tm3.1(cre/ERT2)Bsor expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: A targeting vector was generated with a neomycin resistance cassette, IRES-cre/ERT2 and polyadenylation sequences. The construct was electroporated into ES cells, and homologous recombination inserted the neo-IRES-cre/ERT2-pA sequence into exon 16 of the Abcg2 gene. Correctly targeted ES cells were transiently transfected with a pMC-cre plasmid to excise the nomycin cassette. The ES cells were injected into blastocysts to generate chimeric animals which were used to establish germline transmission. The allele is stated to be hypomorphic for Abcg2 protein expression in the kidney (slight reduction) and liver (greater reduction) determined by Western blot, in homozygous animals.
(J:189206)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Abcg2
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Original: |
J:189206 Fatima S, et al., Abcg2 expression marks tissue-specific stem cells in multiple organs in a mouse progeny tracking model. Stem Cells. 2012 Feb;30(2):210-21 |
| All: |
7 reference(s) |
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Analysis Tools
