Gene Expression Data
Assay Details
Assay
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Reference:
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J:49066
Agulnik SI, et al., Cloning, mapping, and expression analysis of TBX15, a new member of the T-Box gene family. Genomics. 1998 Jul 1;51(1):68-75
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5445586
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Gene symbol:
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Tbx15
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Gene name:
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T-box 15
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Probe:
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Tbx15 cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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The authors reported that they used riboprobes derived from either the 5' end or the 3' end and obtained identical hybridization patterns with each; they did not specify which probe was used for the pictured results.
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Results
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Specimen
5A:
embryonic day 9.5
(more )
Specimen
5A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5B:
embryonic day 10.5
(more )
Specimen
5B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5C:
embryonic day 11.5
(more )
Specimen
5C:
(close )
Genetic Background:
|
Not Specified
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Age:
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embryonic day 11.5
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Sex:
|
Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: forelimb bud |
Present |
Regionally restricted |
5C
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Expression is limited to the medial portion of the limb; it is excluded from the anterior and posterior margins.
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TS19: hindlimb bud |
Present |
Regionally restricted |
5C
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Expression is limited to the medial portion of the limb; it is excluded from the anterior and posterior margins.
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TS19: footplate apical ectodermal ridge |
Absent |
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5C
|
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TS19: handplate apical ectodermal ridge |
Absent |
|
5C
|
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TS19: maxillary process |
Present |
Regionally restricted |
5C
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Expression is present on the lateral, caudal surface.
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TS19: 1st branchial arch |
Present |
Regionally restricted |
5C
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Expression is present in the core of the mandibular process of the first arch.
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TS19: outer ear |
Present |
Regionally restricted |
5C
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Expression outlines the rostral, dorsal edge of the hyomandibular cleft, which will become the external auditory meatus.
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TS19: head |
Strong |
Regionally restricted |
5C
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There is a prominent expression surrounding most the eye; it is strongest at its dorsal edge.
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TS19: visceral organ system |
Absent |
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5C
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No expression was seen in the internal organs.
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Specimen
5D:
embryonic day 12.5
(more )
Specimen
5D:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: forelimb |
Present |
Regionally restricted |
5D
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Expression extends all down the flank. It is lost from the distal limb and becomes more medially restricted.
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TS20: hindlimb |
Present |
Regionally restricted |
5D
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Expression extends all down the flank. It is lost from the distal limb and becomes more medially restricted.
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TS20: mandibular process |
Present |
Regionally restricted |
5D
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Expression is present in portions surrounding the developing mouth.
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TS20: maxillary process |
Present |
Regionally restricted |
5D
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Expression is present in portions surrounding the developing mouth.
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TS20: head |
Strong |
Regionally restricted |
5D
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There is a prominent expression surrounding most the eye; it is strongest at its dorsal edge. There is a conspicuous absence of expression over the surface of the second branchial arch.
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TS20: visceral organ system |
Absent |
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5D
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No expression was seen in the internal organs.
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Specimen
Not shown:
embryonic day 8.5
(more )
Specimen
Not shown:
(close )
Genetic Background:
|
Not Specified
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Age:
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embryonic day 8.5
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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