Antibody:	Anti-Kif21a
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Present	Diffuse	3A	Expression was detected in post-mitotic neurons throughout the central nervous system.
TS17: brain ventricular layer	Present	Diffuse	3A

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present	Diffuse	3B	Expression was detected in the mitotic ventricular zone and in post-mitotic neurons.
TS17: midbrain ventricular layer	Present	Diffuse	3B

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present	Diffuse	3D , 3F	Expression was detected in the midbrain, including Isl1-positive oculomotor neurons.

Structure	Level	Pattern	Image	Note
TS21: midbrain	Present	Diffuse	3I , 3L , 3J , 3G	Expression was detected in the midbrain, including Isl1-positive oculomotor neurons.

Structure	Level	Pattern	Image	Note
TS19: central nervous system	Present	Diffuse	3O , 3M	Expression was detected in the mitotic ventricular zone and in post-mitotic neurons.
TS19: brain ventricular layer	Present	Diffuse	3O , 3M
TS19: neural tube ventricular layer	Present	Diffuse	3O , 3M

Structure	Level	Pattern	Image	Note
TS21: forebrain ventricular layer	Present	Diffuse	3P , 3R
TS21: forebrain	Present	Diffuse	3P , 3R	Expression was detected in post-mitotic mantle zones with only a few areas of negative staining. Higher expression levels were noted in the regions of the habenular commissure and the lateral ventricle.

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present	Diffuse	3S , 3U
TS21: spinal nerve	Present	Regionally restricted	3U , 3S	Expression was detected in spinal motor and sensory nerves.