Probe:	clone p5A7
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS16: head mesenchyme	Present	Single cells		Expression in a few isolated cells underlying the surface ectoderm in the region of the otic vesicle. Expression in one or two melanoblasts ventral to the inner ear.
TS16: optic cup	Strong	Regionally restricted		Expression in a few isolated cells underlying the surface ectoderm in the region of the otic vesicle. Expression in one or two melanoblasts ventral to the inner ear.

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Present	Regionally restricted		Expression in a few melanoblasts in the lateral mesenchyme adjacent to forebrain and hindbrain, but not the midbrain.

Structure	Level	Pattern	Image	Note
TS17: trunk mesenchyme	Present			Expression in only small numbers of melanoblasts in the trunk mesenchyme.
TS17: surface ectoderm	Absent

Structure	Level	Pattern	Image	Note
TS18: head mesenchyme	Present	Regionally restricted		Expression in migratory melanoblasts associated with the eye, in melanoblasts in mesenchyme on all sides of otocyst and around the anterior cardinal vein.

Structure	Level	Pattern	Image	Note
TS19: head mesenchyme	Present	Regionally restricted		Expression in melanoblasts localized to the anterior cardinal vein, particularly near the otic vesicle.

Structure	Level	Pattern	Image	Note
TS19: head mesenchyme	Present	Regionally restricted		Expression in a few isolated melanoblasts localized dorsal and ventral to the eyes, just below or within the ectoderm.

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Present	Regionally restricted		Expression in a stream of melanoblasts between myelencephalon and telencephalon; no expression in mesenchyme near mesencephalon. Colonies and/or clusters of expressing cells in mesenchyme around eye and ear.
TS20: embryo mesenchyme	Present	Regionally restricted		Expression in melanoblasts scattered along the dorsal half of lateral surfaces but not in cells along the dorsal midline.

Structure	Level	Pattern	Image	Note
TS21: head mesenchyme	Present	Regionally restricted		Expression in a stream extending between tmyelencephalon and telencephalon; no expression in the mesenchyme near the mesencephalon. Expression extending along the entire lateral surfaces in many regions of the head, particularly between pinna and eyes.
TS21: embryo mesenchyme	Present	Regionally restricted		Expression in melanoblasts, majority of which lie within mesenchyme. Expression associated with the anterior cardinal vein along its entire length extending from head to heart, and scattered along the posterior cardinal vein.
TS21: trunk mesenchyme	Present	Regionally restricted		Increasingly patchy distribution and number of expressing cells. Large aggregation at the base of hindlimbs.

Structure	Level	Pattern	Image	Note
TS22: head mesenchyme	Present	Regionally restricted		Expression in melanoblasts in mesenchyme surrounding the mesencephalon, eye, and ear.
TS22: trunk mesenchyme	Present	Regionally restricted		No expression along the dorsal midline of the head or trunk.
TS22: skin	Present	Regionally restricted		Widespread expression in ectoderm, but no expression in some areas of developing skin, especially in the trunk.

Structure	Level	Pattern	Image	Note
TS27: pigmented retinal epithelium	Strong	Uniform		Intense uniform expression in the entire retinal pigmented epithelium, locally thickened in patches on the convex surface, probably representing choroidal melanocytes.
TS27: ciliary body	Present
TS27: Harderian gland	Present	Regionally restricted		Expression in numerous dendritic melanocytes.
TS27: semicircular duct ampulla	Present	Regionally restricted		Expression in melanocytes within the stria vascularis of each cochlear turn and within the utricle, crus commune and ampullae of the vestibular region
TS27: stria vascularis	Present	Regionally restricted		Expression in melanocytes within the stria vascularis of each cochlear turn and within the utricle, crus commune and ampullae of the vestibular region
TS27: utricle	Present	Regionally restricted		Expression in melanocytes within the stria vascularis of each cochlear turn and within the utricle, crus commune and ampullae of the vestibular region
TS27: inner ear vestibular component	Present	Regionally restricted		Expression in melanocytes within the stria vascularis of each cochlear turn and within the utricle, crus commune and ampullae of the vestibular region

Structure	Level	Pattern	Image	Note
TS21: pinna mesenchyme	Present	Regionally restricted	2A	Expression in melanoblasts aggregated within the surface ectoderm and underlying mesenchyme of the pinna.
TS21: pinna surface epithelium	Present	Regionally restricted	2A	Expression in melanoblasts aggregated within the surface ectoderm and underlying mesenchyme of the pinna.

Structure	Level	Pattern	Image	Note
TS21: trunk mesenchyme	Present	Regionally restricted	2B	Expression in a large aggregation of melanoblasts at the base of the tail lying adjacent to the hindlimb buds.

Structure	Level	Pattern	Image	Note
TS21: trunk mesenchyme	Present	Regionally restricted	2C	Expression in clusters of melanoblasts at the base of the tail.

Structure	Level	Pattern	Image	Note
TS27: dermis	Present	Widespread	2D	Expression in large number of melanocytes uniformly distributed within the dermis and hair follicles of the skin.
TS27: hair follicle	Present	Widespread	2D	Expression in large number of melanocytes uniformly distributed within the dermis and hair follicles of the skin.

Structure	Level	Pattern	Image	Note
TS27: skin	Absent		2E

Structure	Level	Pattern	Image	Note
TS15: optic cup	Present	Regionally restricted	3A	Expression in the pigmented retinal epithelium of the optic vesicle, localized along the ventral medial edge of the optic vesicle.

Structure	Level	Pattern	Image	Note
TS16: optic cup	Present	Regionally restricted	3B	Expression in the pigmented retinal epithelium of the optic vesicle.

Structure	Level	Pattern	Image	Note
TS19: pigmented retinal epithelium	Present	Widespread	3C	Expression across the entire width of the pigmented retinal epithelium.

Structure	Level	Pattern	Image	Note
TS19: pigmented retinal epithelium	Present	Widespread	3D	Expression across the entire width of the pigmented retinal epithelium.

Structure	Level	Pattern	Image	Note
TS21: pigmented retinal epithelium	Present		3E
TS21: head mesenchyme	Present	Regionally restricted	3E	Expression in migratory melanoblasts.

Structure	Level	Pattern	Image	Note
TS21: head mesenchyme	Present	Regionally restricted	3F	Expression in a few migratory melanoblasts.

Structure	Level	Pattern	Image	Note
TS22: eyelid	Present	Regionally restricted	3G	Expression in numerous melanoblasts in the skin of developing eyelids.
TS22: eye	Present	Regionally restricted	3G	Expression in migratory melanoblasts extending in a ventral-medial direction from the pigmented retinal epithelium, probably delineate the Harderian gland.
TS22: optic choroid	Present	Regionally restricted	3G	Local thickenings of expression around the outer edge of the pigmented retinal epithelium, may represent choroidal melanoblasts.

Structure	Level	Pattern	Image	Note
TS22: eye	Present	Regionally restricted	3H	Expression in isolated melanoblasts in the mesenchyme surrounding region of expression in the pigmented retinal epithelium.

Structure	Level	Pattern	Image	Note
TS19: head mesenchyme	Present	Regionally restricted	4A	Expression in migratory melanoblasts around the dorsal and lateral edges of the otic vesicle and anterior cardinal vein.

Structure	Level	Pattern	Image	Note
TS19: inner ear mesenchyme	Present	Regionally restricted	4B	Expression in melanoblasts with a more restricted distribution compared with control embryos.

Structure	Level	Pattern	Image	Note
TS19: inner ear mesenchyme	Present	Regionally restricted		The difference in melanoblast density compared with controls becomes even more apparent.

Structure	Level	Pattern	Image	Note
TS19: inner ear mesenchyme	Present	Regionally restricted		Within the inner ear, the density of expressing melanoblast is lower than in controls.

Structure	Level	Pattern	Image	Note
TS19: inner ear mesenchyme	Present	Single cells	4C	Expression in two cells dorsal to the otic vesicle.

Structure	Level	Pattern	Image	Note
TS19: inner ear mesenchyme	Present			The difference in melanoblast density compared with controls becomes even more apparent.

Structure	Level	Pattern	Image	Note
TS20: inner ear mesenchyme	Present	Regionally restricted	4D	Expression in melanoblasts localized to the ventral part of the otic vesicle, which is differentiating into the cochlear duct.

Structure	Level	Pattern	Image	Note
TS19: inner ear mesenchyme	Present			Difference in melanoblast density compared with controls.

Structure	Level	Pattern	Image	Note
TS20: ear	Absent		4E

Structure	Level	Pattern	Image	Note
TS21: inner ear mesenchyme	Present	Regionally restricted	4F	Expression in melanoblast localized mainly to the mesenchyme adjacent to the portion of the cochlear duct which later differentiates into the stria vascularis.

Structure	Level	Pattern	Image	Note
TS21: inner ear mesenchyme	Absent		4G	No expressing cells are detected in the mesenchyme surrounding the cochlea.

Structure	Level	Pattern	Image	Note
TS22: inner ear mesenchyme	Present	Single cells		Expression in relatively few melanoblasts in mesenchyme, developing skin, or associated with the eye or inner ear.