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Pvalbtm1.1(cre/ERT2)Hze
Targeted Allele Detail
Summary
Symbol: Pvalbtm1.1(cre/ERT2)Hze
Name: parvalbumin; targeted mutation 1.1, Hongkui Zeng
MGI ID: MGI:5461312
Synonyms: Pvalb-2A-CreERT2, Pvalb-2A-CreERT2-Deltahygro, Pvalbtm1.1(cre/ERT2)Htz
Gene: Pvalb  Location: Chr15:78075314-78090600 bp, - strand  Genetic Position: Chr15, 36.93 cM, cytoband E
Alliance: Pvalbtm1.1(cre/ERT2)Hze page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:191262
Parent Cell Line:  G4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NCrl)F1
Mutation
description
Allele Type:    Targeted (Inducible, Recombinase)
Inducer:    tamoxifen
Mutation:    Insertion
 
Pvalbtm1.1(cre/ERT2)Hze expression driven by 1 gene
 
Mutation details: Embryonic stem (ES) cells, previously targeted with a T2A-Cre vector immediately downstream of the parvalbumin translational STOP codon were re-targeted with a T2A-CreERT2 vector and a FLP-expressing plasmid to facilitate recombination. Correctly targeted ES cells had (from 5' to 3') a three amino acid linker sequence, an frt3 site within Pvalb intron 3, a partial Pvalb exon 4 sequence up to (but not including) the endogenous stop codon, a T2A sequence that is in-frame with the Pvalb coding sequence, a CreERT2 fusion gene, the Pvalb 3' UTR sequence from exon 4, an attB site, a PGK-hygromycin-SV40polyA cassette and an attP site. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were bred to PhiC31-expressing mice to delete the attB/attP-flanked PGK cassette. The resulting mice were bred with C57BL/6J wildtype mice for at least two additional generations (and the PhiC31 transgene was removed). (J:155793, J:191262, J:220523)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Pvalb (mouse)
Summary of all recombinase alleles driven by Pvalb.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pvalb Mutation:  29 strains or lines available
References
Original:  J:220523 Harris JA, et al., Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits. 2014;8:76
All:  7 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/19/2024
MGI 6.24 		The Jackson Laboratory