Pvalbtm2.1(flpe)Hze
Targeted Allele Detail
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| Symbol: |
Pvalbtm2.1(flpe)Hze |
| Name: |
parvalbumin; targeted mutation 2.1, Hongkui Zeng |
| MGI ID: |
MGI:5461313 |
| Synonyms: |
Pvalb-2A-Flpe, Pvalb-2A-FlpE-Deltahygro |
| Gene: |
Pvalb Location: Chr15:78075314-78090600 bp, - strand Genetic Position: Chr15, 36.93 cM, cytoband E
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| Alliance: |
Pvalbtm2.1(flpe)Hze page
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| Allele Type: |
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Targeted (Recombinase) |
| Mutation: |
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Insertion
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Pvalbtm2.1(flpe)Hze expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Pvalb (MGI:97821) |
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Mutation details: Embryonic stem (ES) cells, previously targeted with a T2A-Cre vector immediately downstream of the parvalbumin translational STOP codon were re-targeted with a T2A-FLPe (enhanced flippase) vector and a FLP-expressing plasmid to facilitate recombination. Correctly targeted ES cells had (from 5' to 3') a three amino acid linker sequence, an frt3 site within Pvalb intron 3, a partial Pvalb exon 4 sequence up to (but not including) the endogenous stop codon, a T2A sequence that is in-frame with the Pvalb coding sequence, a sequence-enhanced flippase gene (FLPe), the Pvalb 3' UTR sequence from exon 4, an attB site, a PGK-hygromycin-SV40polyA cassette and an attP site. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were bred to PhiC31-expressing mice to delete the attB/attP-flanked PGK cassette. The resulting mice were bred with C57BL/6J wildtype mice for at least two additional generations (and the PhiC31 transgene was removed).
(J:155793, J:191264, J:219930)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Pvalb
(mouse)
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| Original: |
J:219930 Madisen L, et al., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron. 2015 Mar 4;85(5):942-58 |
| All: |
9 reference(s) |
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Analysis Tools
