Pvalbtm3.1(dreo)Hze
Targeted Allele Detail
|
|
| Symbol: |
Pvalbtm3.1(dreo)Hze |
| Name: |
parvalbumin; targeted mutation 3.1, Hongkui Zeng |
| MGI ID: |
MGI:5461318 |
| Synonyms: |
Pvalb-2A-Dre, Pvalb-2A-DreO-Deltahygro, PvalbDre |
| Gene: |
Pvalb Location: Chr15:78075314-78090600 bp, - strand Genetic Position: Chr15, 36.93 cM, cytoband E
|
| Alliance: |
Pvalbtm3.1(dreo)Hze page
|
|
|
|
| Allele Type: |
|
Targeted (Recombinase) |
| Mutation: |
|
Insertion
|
| |
|
Pvalbtm3.1(dreo)Hze expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Pvalb (MGI:97821) |
|
|
| |
|
Mutation details: ES cells containing Pvalbtm1.1(cre)Aibs were retargeted to insert a viral 2A oligopeptide (T2A) that mediates ribosomal skipping and a mammalian-optimized D6 site-specific DNA recombinase (dreo recombinase) inserted immediately downstream of the parvalbumin translational STOP codon. Correctly targeted ES cells had (from 5' to 3') a three amino acid linker sequence, an frt3 site within Pvalb intron 3, a partial Pvalb exon 4 sequence up to (but not including) the endogenous stop codon, a T2A sequence that is in-frame with the Pvalb coding sequence, a D6 site-specific DNA recombinase optimized for mammalian cell expression (DreO recombinase), the Pvalb 3' UTR sequence from exon 4, an attB site, a PGK-hygromycin-SV40polyA cassette (with an RNA spice donor-frt5 site-RNA spice acceptor in the hygromycin gene), and an attP site. PhiC31-mediated recombination removed the hygro cassette.
(J:191266, J:219930)
|
|
|
|
Activity: |
 Tissue activity of this recombinase allele
|
|
Driver:
|
Pvalb
(mouse)
|
| |
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
|
|
| Original: |
J:219930 Madisen L, et al., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron. 2015 Mar 4;85(5):942-58 |
| All: |
8 reference(s) |
|
Analysis Tools
