H3f3atm1.2Mnn
Targeted Allele Detail
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| Symbol: |
H3f3atm1.2Mnn |
| Name: |
H3.3 histone A; targeted mutation 1.2, Jeff Mann |
| MGI ID: |
MGI:5474377 |
| Gene: |
H3f3a Location: Chr1:180630125-180641127 bp, - strand Genetic Position: Chr1, 84.46 cM, cytoband D2.3
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| Alliance: |
H3f3atm1.2Mnn page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:194069
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| Parent Cell Line: |
2A (ES Cell)
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| Strain of Origin: |
129S1/SvImJ
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| Allele Type: |
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Targeted (Null/knockout, Reporter) |
| Mutations: |
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Insertion, Intragenic deletion
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Mutation details: A targeting vector was designed to contain, from 5' to 3', a synthetic splice acceptor, a loxP site, an ATG start codon, a wild-type H3 histone isoform 3 (H3.3) coding sequence, H3f3a (H3 histone, family 3A) 3' UTR, and an SV40 polyadenylation (polyA) sequence. This was followed by a downstream frt-flanked neomycin resistance (neo) cassette, and a second loxP site, an ATG start codon, the venus variant of yellow fluorescent protein (YFP), and another SV40 polyA sequence. This vector replaced part of exon 2. Flp-mediated recombination removed the neo cassette. Cre-mediated recombination removed the H3.3 isoform and allowed for expression of the reporter gene.
(J:194069)
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Targeting vectors for the generation of the H3f3atm1.1Mnn, H3f3atm1.2Mnn, H3f3btm1.1Mnn and H3f3btm1.2Mnn alleles |
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any H3f3a Mutation: |
29 strains or lines available
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| Original: |
J:194069 Tang MC, et al., Conditional allelic replacement applied to genes encoding the histone variant H3.3 in the mouse. Genesis. 2013 Feb;51(2):142-6 |
| All: |
3 reference(s) |
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Analysis Tools
