Summary | Mutation origin | Mutation description | Expression | Recombinase activity | Phenotypes | Disease models | Find Mice (IMSR) | References

Symbol:	Chattm2(cre)Lowl
Name:	choline O-acetyltransferase; targeted mutation 2, Bradford B Lowell
MGI ID:	MGI:5475195
Synonyms:	ChAT-IRES-Cre
Gene:	Chat  Location: Chr14:32130160-32187866 bp, - strand  Genetic Position: Chr14, 19.4 cM
Alliance:	Chattm2(cre)Lowl page

Germline Transmission:	Earliest citation of germline transmission: J:169562
Parent Cell Line:	W4 (ES Cell)
Strain of Origin:	129S6/SvEvTac

Allele Type:    Targeted (Recombinase) Mutation:    Insertion   Chattm2(cre)Lowl expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Chat (MGI:88392)   Mutation details: An optimized internal ribosome entry sequence (IRES) fused to cre recombinase followed by a neomycin resistance cassette flanked FRT sites was inserted after the ChAT stop codon in BAC (bMQ185k21) using homologous recombination in bacteria. A genomic ChAT fragment with 4kb homology arms on either side of the IRES-Cre insertion site was lifted out from the BAC using reverse recombineering techniques and transformed into ES cells. This allele was used to generate Chattm1(cre)Lowl. (J:169562)

Activity:	Tissue activity of this recombinase allele
Driver:	Chat (mouse) Summary of all recombinase alleles driven by Chat. Comparative matrix view of recombinase activities Alliance mouse Chat gene page

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:

2 RNA-Seq or microarray experiment(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 4 strains available      Cell Lines: 0 lines available
Carrying any Chat Mutation:	58 strains or lines available

Original:	J:169562 Rossi J, et al., Melanocortin-4 receptors expressed by cholinergic neurons regulate energy balance and glucose homeostasis. Cell Metab. 2011 Feb 2;13(2):195-204
All:	384 reference(s)