Gt(ROSA)26Sortm3.1(RARE-CFP)Mgn
Targeted Allele Detail
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| Symbol: |
Gt(ROSA)26Sortm3.1(RARE-CFP)Mgn |
| Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 3.1, Mark A Magnussen |
| MGI ID: |
MGI:5476515 |
| Synonyms: |
-60/DR5-TA-CFP |
| Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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| Alliance: |
Gt(ROSA)26Sortm3.1(RARE-CFP)Mgn page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:195112
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| Parent Cell Line: |
Not Specified (ES Cell)
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| Strain of Origin: |
129S6/SvEvTac
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| Allele Type: |
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Targeted (RMCE-ready, Reporter) |
| Mutation: |
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Insertion
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Mutation details: This allele was created by RMCE in Gt(ROSA)26Sortm1Mgn. The original selections cassettes (puromycin N-acetyltransferase and a truncated form of HSV1 thymidine kinase and EM7-driven neomycin resistance) were replaced by a cassette composed of ROSA26 upstream sequence deleted from the start codon to -60, 5X direct repeat of a DR5 (AGGTCAccaggAGGTCA) which is a consensus RARE controlling expression of the CFP reporter. An upstream FLP flanked hygromycin selection cassette was subsequently removed by flp recombinase.
(J:195112)
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| Original: |
J:195112 Serup P, et al., Partial promoter substitutions generating transcriptional sentinels of diverse signaling pathways in embryonic stem cells and mice. Dis Model Mech. 2012 Nov;5(6):956-66 |
| All: |
1 reference(s) |
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Analysis Tools
