About   Help   FAQ
Gt(ROSA)26Sortm1(creB)Dwi
Targeted Allele Detail
Summary
Symbol: Gt(ROSA)26Sortm1(creB)Dwi
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Douglas J Winton
MGI ID: MGI:5486456
Synonyms: Rosa26-creB
Gene: Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance: Gt(ROSA)26Sortm1(creB)Dwi page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:194841
Parent Cell Line:  S6B6 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6J)F1
Mutation
description
Allele Type:    Targeted (Recombinase)
Mutation:    Insertion
 
Gt(ROSA)26Sortm1(creB)Dwi expression driven by 1 gene
 
Mutation details: A cassette was constructed with the creB fragment of cre recombinase (codons 60-343) fused to an FKBP dimerization domain by an F2 linker and included a polyadenylation sequence. This cassette was cloned into a plasmid containing Gt(ROSA)26Sor homology sequences. The linearized plasmid was introduced into ES cells and by homologous recombination the creB sequence was inserted into the Gt(ROSA)26Sor locus. When crossed to mice expressing the creA sequence, the two fragments will dimerize in presence of AP20187 and a functional cre protein will be produced. (J:194841)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Gt(ROSA)26Sor (mouse)
Summary of all recombinase alleles driven by Gt(ROSA)26Sor.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  993 strains or lines available
References
Original:  J:194841 Buczacki SJ, et al., Intestinal label-retaining cells are secretory precursors expressing Lgr5. Nature. 2013 Mar 7;495(7439):65-9
All:  1 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory