T<tm1(cre/ERT2)Hssk> Targeted Allele Detail MGI Mouse (MGI:5490031)

T^{tm1(cre/ERT2)Hssk}
Targeted Allele Detail

Summary

Symbol:	T^{tm1(cre/ERT2)Hssk}
Name:	brachyury, T-box transcription factor T; targeted mutation 1, Hiroshi Sasaki
MGI ID:	MGI:5490031
Synonyms:	T^EGFP, T^{nEGFP-CreERT2}
Gene:	T Location: Chr17:8653255-8661328 bp, + strand Genetic Position: Chr17, 4.92 cM
Alliance:	T^{tm1(cre/ERT2)Hssk} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:194054
Parent Cell Line:	TT2 (ES Cell)
Strain of Origin:	(C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation
description

Allele Type:

Targeted (Inducible, Recombinase)

Inducer:

tamoxifen

Mutations:

Insertion, Intragenic deletion

T^{tm1(cre/ERT2)Hssk} expression driven by 1 gene

Mutation details: Recombineering of the BAC clone RP23-99E8 containing the T (brachyury) locus generated a targeting vector designed to replace the stop codon of T and introduce sequences encoding the ERAV 2A peptide, a human histone H2B/EGFP fusion protein (protein is expressed in the cell nucleus), the Thosea asigna virus 2A peptide and cre/ERT2 fusion protein. A self-excising ACN cassette (containing the neo resistance marker) was also included. The vector was electroporated into ES cells for homologous recombination. Chimeras were generated from 3 independent clones (36, 41, and 44) and were bred to C57BL/6J females, with the ACN cassette self-excising during passage through the germline. The line derived from #41 was analyzed further. (J:194054)

Recombinase
activity

Activity:	Tissue activity of this recombinase allele
Driver:	T (mouse) Summary of all recombinase alleles driven by T. Comparative matrix view of recombinase activities Alliance mouse T gene page

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:

17 assay results

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available Cell Lines: 0 lines available
Carrying any T Mutation:	59 strains or lines available

References

Original:	J:194054 Imuta Y, et al., Generation of knock-in mice that express nuclear enhanced green fluorescent protein and tamoxifen-inducible Cre recombinase in the notochord from Foxa2 and T loci. Genesis. 2013 Mar;51(3):210-8
All:	9 reference(s)

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