Pvalbtm4.1(flpo)Hze
Targeted Allele Detail
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| Symbol: |
Pvalbtm4.1(flpo)Hze |
| Name: |
parvalbumin; targeted mutation 4.1, Hongkui Zeng |
| MGI ID: |
MGI:5490601 |
| Synonyms: |
Pvalb-2A-Flpo, Pvalb-2A-FlpO-deltaHygro |
| Gene: |
Pvalb Location: Chr15:78075314-78090600 bp, - strand Genetic Position: Chr15, 36.93 cM, cytoband E
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| Alliance: |
Pvalbtm4.1(flpo)Hze page
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| Allele Type: |
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Targeted (Recombinase) |
| Mutation: |
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Insertion
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Pvalbtm4.1(flpo)Hze expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Pvalb (MGI:97821) |
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Mutation details: ES cells already targeted with a T2A-Cre vector immediately downstream of the parvalbumin translational STOP codon, were re-targeted with a "T2A-Flpo" vector and a FLP-expressing plasmid to facilitate recombination. Correctly targeted ES cells had (from 5' to 3') a three amino acid linker sequence, an frt3 site within Pvalb intron 3, a partial Pvalb exon 4 sequence up to (but not including) the endogenous stop codon, a T2A sequence in-frame with the Pvalb coding sequence, a FlpO recombinase gene (a codon-optimized FLPe modified for translation in mammalian cells and higher recombination efficiency), the Pvalb 3' UTR sequence from exon 4, an attB site, a PGK-hygromycin-SV40polyA cassette (with a frt5 site in the hygromycin gene), and an attP site. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were bred to PhiC31-expressing mice (C57BL/6J congenic background; see JAX Stock No. 007743) to delete the attB/attP-flanked PGK-hygromycin-polyA cassette. Heterozygous mice have an FlpO recombinase expression pattern in the brain similar to endogenous Pvalb expression.
(J:197021, J:219930)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Pvalb
(mouse)
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| Original: |
J:219930 Madisen L, et al., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron. 2015 Mar 4;85(5):942-58 |
| All: |
31 reference(s) |
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Analysis Tools
