Epxtm1.1(cre)Jlee
Targeted Allele Detail
|
|
| Symbol: |
Epxtm1.1(cre)Jlee |
| Name: |
eosinophil peroxidase; targeted mutation 1.1, James J Lee |
| MGI ID: |
MGI:5498555 |
| Synonyms: |
eoCRE |
| Gene: |
Epx Location: Chr11:87754826-87766362 bp, - strand Genetic Position: Chr11, 52.25 cM
|
| Alliance: |
Epxtm1.1(cre)Jlee page
|
|
| Germline Transmission: |
Earliest citation of germline transmission:
J:198489
|
| Parent Cell Line: |
Not Specified (ES Cell)
|
| Strain of Origin: |
C57BL/6J
|
|
| Allele Type: |
|
Targeted (Recombinase) |
| Mutation: |
|
Insertion
|
| |
|
Epxtm1.1(cre)Jlee expression driven by
1 gene
Knock-in expression driven by:
|
| |
|
Mutation details: A sequence encoding a mammalian codon-optimized cre recombinase was inserted at the start codon of the Epx locus by homologous recombination. An IRES was inserted before the coding exons of Epx, so that a dicstronic mRNA encoding cre and Epx would be generated. A neo selection cassette was included in the original construct, and was subsequently removed from the targeted allele by a backcross to a flp deleter mouse strain. It was determined that Epx expression was not rescued efficienty from the modified allele by the IRES. Flow cytometric GFP analyses of cell populations from offspring of eoCre mice and reporter animals demonstrated that cre expression is observed only in eosinophils.
(J:198489)
|
|
|
|
Activity: |
 Tissue activity of this recombinase allele
|
|
Driver:
|
Epx
(mouse)
|
| |
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
| Carrying any Epx Mutation: |
32 strains or lines available
|
|
| Original: |
J:198489 Doyle AD, et al., Homologous recombination into the eosinophil peroxidase locus generates a strain of mice expressing Cre recombinase exclusively in eosinophils. J Leukoc Biol. 2013 Jul;94(1):17-24 |
| All: |
22 reference(s) |
|
Analysis Tools
