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Penktm1.1(cre/ERT2)Hze
Targeted Allele Detail
Summary
Symbol: Penktm1.1(cre/ERT2)Hze
Name: preproenkephalin; targeted mutation 1.1, Hongkui Zeng
MGI ID: MGI:5501235
Synonyms: Penk-2A-CreERT2, Penk-2A-CreERT2-DeltaNeo
Gene: Penk  Location: Chr4:4133536-4138815 bp, - strand  Genetic Position: Chr4, 2.31 cM
Alliance: Penktm1.1(cre/ERT2)Hze page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:199217
Parent Cell Line:  G4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NCrl)F1
Mutation
description
Allele Type:    Targeted (Inducible, Recombinase)
Inducer:    tamoxifen
Mutations:    Insertion, Intragenic deletion
 
Penktm1.1(cre/ERT2)Hze expression driven by 1 gene
 
Mutation detailsA targeting vector contained a partial Penk intron 1 sequence containing an frt3 site, a partial Penk exon 2 sequence up to (but not including) the endogenous stop codon, a self-cleaving T2A sequence in-frame with the Nxph4 coding sequence, a cre/ERT2 fusion gene, a bovine growth hormone polyA signal, an AttB site, a PGK-Neo-polyA cassette, an frt5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 polyA signal, an AttP site and genomic sequence from the Penk locus starting 509 bp downstream of the endogenous stop codon (to create a 511 bp deletion including, the stop codon). This targeting vector was electroporated into G4 ES cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were bred to PhiC31-expressing mice to delete the AttB/AttP-flanked sequences and replace it with the recombined AttB/AttP site (AttL). (J:199217, J:220523)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Penk (mouse)
Summary of all recombinase alleles driven by Penk.
 

Expression
In Mice Carrying this Mutation: 5 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Penk Mutation:  21 strains or lines available
References
Original:  J:220523 Harris JA, et al., Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits. 2014;8:76
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
07/02/2024
MGI 6.13
The Jackson Laboratory