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Trib2tm1.1(cre/ERT2)Hze
Targeted Allele Detail
Summary
Symbol: Trib2tm1.1(cre/ERT2)Hze
Name: tribbles pseudokinase 2; targeted mutation 1.1, Hongkui Zeng
MGI ID: MGI:5501236
Synonyms: Trib2-2A-CreERT2, Trib2-2A-CreERT2-DeltaNeo/Hygro
Gene: Trib2  Location: Chr12:15841728-15866923 bp, - strand  Genetic Position: Chr12, 7.25 cM
Alliance: Trib2tm1.1(cre/ERT2)Hze page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:199218
Parent Cell Line:  G4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NCrl)F1
Mutation
description
Allele Type:    Targeted (Inducible, Recombinase)
Inducer:    tamoxifen
Mutations:    Insertion, Intragenic deletion
 
Trib2tm1.1(cre/ERT2)Hze expression driven by 1 gene
 
Mutation details: ES cells were re-targeted with an "F2A-CreERT2" vector and an FLP-expressing plasmid to facilitate recombination. Correctly re-targeted ES cells had (from 5'to3') an frt3 site within Trib 2 intron 1, a self-cleaving 2A peptide sequence designed to splice a cre/ERT2 fusion gene into a frame with the Trib 2 exon 1 sequence, a bovine growth hormone polyA signal, an AttB site, a PGK-hygromycin-SV40polyA cassette (with a splice donor-frt5 site-RNA splice acceptor in the hygromycin gene), an AttP site, and Trib 2 exon 2 deleted. The resulting chimeric animals were bred to PhiC31-expressing mice to delete the AttB/AttP-flanked sequences and replace them with the recombined AttB/AttP site (AttL). (J:199218, J:220523)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Trib2 (mouse)
Summary of all recombinase alleles driven by Trib2.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Trib2 Mutation:  29 strains or lines available
References
Original:  J:220523 Harris JA, et al., Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits. 2014;8:76
All:  12 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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11/12/2024
MGI 6.24 		The Jackson Laboratory