Rasgrf2<tm1(cre/folA)Hze> Targeted Allele Detail MGI Mouse (MGI:5501498)

Rasgrf2^{tm1(cre/folA)Hze}
Targeted Allele Detail

Summary

Symbol:	Rasgrf2^{tm1(cre/folA)Hze}
Name:	RAS protein-specific guanine nucleotide-releasing factor 2; targeted mutation 1, Hongkui Zeng
MGI ID:	MGI:5501498
Synonyms:	Rasgrf2-2A-dCre, Rasgrf2-2A-DHFR-Cre, Rasgrf2-2A-ecDHFR^R12Y/Y100I/Cre, Rasgrf2-2A-ecDHFRR12Y/Y100I/Cre, Rasgrf2-2A-hDHFR/Cre
Gene:	Rasgrf2 Location: Chr13:92028519-92268164 bp, - strand Genetic Position: Chr13, 47.43 cM, cytoband C3-D1
Alliance:	Rasgrf2^{tm1(cre/folA)Hze} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:199220
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Mutation
description

Allele Type:

Targeted (Inducible, Recombinase)

Inducer:

trimethoprim

Mutation:

Insertion

Rasgrf2^{tm1(cre/folA)Hze} expression driven by 1 gene

Mutation details: The targeting vector containied partial Rasgrf2 intron 29-33 sequence containing an frt3 site in intron 33, a partial Rasgrf2 exon 34 sequence up to (but not including) the endogenous stop codon, self-splicing T2A sequence in-frame with the Rasgrf2 coding sequence, a dCre fusion gene in-frame with the Rasgrf2 coding sequence, a bovine growth hormone polyA signal, an AttB site, a PGK-Neo-polyA cassette, an frt5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 polyA signal, and an AttP site. The dCre fusion gene (destabilized Cre, hDHFR/Cre or ecDHFR/Cre) is Cre recombinase fused at its N terminus to the first 159 amino acids of the E. coli K-12 strain chromosomal dihydrofolate reductase gene (DHFR or folA) harboring the G67S mutation and modified to include the R12Y/Y100I destabilizing domain mutations. The targeting vector was electroporated into G4 ES cells. Correctly targeted ES cells were injected into recipient blastocysts. When treated with trimethoprim, cre reporter gene activity is observed at high levels in cortical layers 2/3 and other scattered cells of the cortex, hypothalamus, thalamus, and midbrain. (J:199220, J:220523)

Recombinase
activity

Activity:	Tissue activity of this recombinase allele
Driver:	Rasgrf2 (mouse) Summary of all recombinase alleles driven by Rasgrf2. Comparative matrix view of recombinase activities Alliance mouse Rasgrf2 gene page

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Rasgrf2 Mutation:	73 strains or lines available

References

Original:	J:220523 Harris JA, et al., Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits. 2014;8:76
All:	25 reference(s)

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