Results
|
Specimen
not shown:
embryonic day 11.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: retina |
Present |
|
|
Expression was detected in the nuclei.
|
Specimen
1A:
embryonic day 12.5
(more )
Specimen
1A:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: retina |
Present |
Regionally restricted |
1A
|
Expression was detected in both the ganglion cell layer and the neuroblast layer.
|
Specimen
1B:
embryonic day 14.5
(more )
Specimen
1B:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: retina |
Present |
Regionally restricted |
1B
|
Expression was detected in both the ganglion cell layer and the neuroblast layer.
|
Specimen
1C:
embryonic day 16.5
(more )
Specimen
1C:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: retina |
Present |
Regionally restricted |
1C
|
Expression was detected mainly in the ganglion cell layer.
|
Specimen
1D:
postnatal day 0
(more )
Specimen
1D:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
postnatal day 0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: retina ganglion cell layer |
Present |
|
1D
|
Expression in the ganglion cell layer was still high at P0.
|
TS27: retina layer |
Present |
Regionally restricted |
1D
|
Expression was detected in a row of regularly spaced cells located in the outer part of the retina.
|
Specimen
1E:
postnatal day 5
(more )
Specimen
1E:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
postnatal day 5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: retina ganglion cell layer |
Weak |
|
1E
|
Expression in the ganglion cell layer decreased markedly at P5.
|
TS28: retina layer |
Present |
Regionally restricted |
1E
|
Expression was detected in a row of regularly spaced cells located in the outer part of the retina.
|
Specimen
1F:
postnatal day 16
(more )
Specimen
1F:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
postnatal day 16
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
not shown:
embryonic day 11.5; Atoh7tm1.1Xmu/?
(more )
Note: Also stained for Atoh7 reporter (assay MGI:5509320). This specimen could have been either heterozygous or homozygous for Math5HA.
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac
|
Mutant Allele(s):
|
Atoh7tm1.1Xmu/?
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Also stained for Atoh7 reporter (assay MGI:5509320). This specimen could have been either heterozygous or homozygous for Math5HA.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: retina |
Present |
Regionally restricted |
|
There was almost complete overlap of expression with Math5 in the neuroblast layer of the central retina.
|
Specimen
3A,A'',A''':
embryonic day 12.5; Atoh7tm1.1Xmu/?
(more )
Note: Double labeled: green - Onecut1; magenta - Atoh7 (assay MGI:5509320). This specimen could have been either heterozygous or homozygous for Math5HA.
Specimen
3A,A'',A''':
(close )
Genetic Background:
|
involves: 129S6/SvEvTac
|
Mutant Allele(s):
|
Atoh7tm1.1Xmu/?
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Onecut1; magenta - Atoh7 (assay MGI:5509320). This specimen could have been either heterozygous or homozygous for Math5HA.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: retina |
Present |
Regionally restricted |
3A''
,
3A
,
3A'''
|
There was almost complete overlap of expression with Math5 in the neuroblast layer of the central retina. Expression was also detected in the ganglion cell layer.
|
Specimen
3B,B'',B''':
embryonic day 14.5; Atoh7tm1.1Xmu/?
(more )
Note: Double labeled: green - Onecut1; magenta - Atoh7 (assay MGI:5509320). This specimen could have been either heterozygous or homozygous for Math5HA.
Specimen
3B,B'',B''':
(close )
Genetic Background:
|
involves: 129S6/SvEvTac
|
Mutant Allele(s):
|
Atoh7tm1.1Xmu/?
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Onecut1; magenta - Atoh7 (assay MGI:5509320). This specimen could have been either heterozygous or homozygous for Math5HA.
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: retina |
Present |
Regionally restricted |
3B'''
,
3B
,
3B''
|
Expression was detected in the ganglion cells layer. In the neuroblast layer, the overlap with Math5 was significantly reduced.
|
Specimen
3C,C'',C''':
embryonic day 12.5
(more )
Note: Double labeled: green - Onecut1; magenta - Pou4f2 (assay MGI:5509319).
Specimen
3C,C'',C''':
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Onecut1; magenta - Pou4f2 (assay MGI:5509319).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: retina |
Present |
Regionally restricted |
3C''
,
3C'''
,
3C
|
All retinal ganglion cells in the ganglion cell layer expressed both Pou4f2 and Oc1. In the neuroblast layer Pou4f2-positive cells expressed Oc1, but only a subset of Oc1-positive cells also expressed Pou4f2.
|
Specimen
3D,D'',D''':
embryonic day 14.5
(more )
Note: Double labeled: green - Onecut1; magenta - Pou4f2 (assay MGI:5509319).
Specimen
3D,D'',D''':
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Onecut1; magenta - Pou4f2 (assay MGI:5509319).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: retina |
Present |
Regionally restricted |
3D'''
,
3D''
,
3D
|
All retinal ganglion cells in the ganglion cell layer expressed both Pou4f2 and Oc1. Overlap of expression in the neuroblast layer decreased.
|
Specimen
not shown E12.5:
embryonic day 12.5
(more )
Note: Also stained for Isl1 (assay MGI:5509329).
Specimen
not shown E12.5:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Also stained for Isl1 (assay MGI:5509329).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: retina |
Present |
Regionally restricted |
|
There was overlap of expression with Isl1 in both the ganglion cell layer and the neuroblast layer.
|
Specimen
not shown E14.5:
embryonic day 14.5
(more )
Note: Also stained for Isl1 (assay MGI:5509329).
Specimen
not shown E14.5:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Also stained for Isl1 (assay MGI:5509329).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: retina |
Present |
Regionally restricted |
|
There was overlap of expression with Isl1 in both the ganglion cell layer and the neuroblast layer.
|
Specimen
6B,B',B''':
embryonic day 12.5
(more )
Note: Double labeled: green - Onecut1; magenta - Pcna (assay MGI:5509378).
Specimen
6B,B',B''':
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Onecut1; magenta - Pcna (assay MGI:5509378).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: retina |
Present |
Regionally restricted |
6B'''
,
6B'
,
6B
|
Most expressing cells in the neuroblast layer were also PCNA-positive, but some cells were PCNA-negative.
|
Specimen
6E,E',E''':
embryonic day 14.5
(more )
Note: Double labeled: green - Onecut1; magenta - Pcna (assay MGI:5509378).
Specimen
6E,E',E''':
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Onecut1; magenta - Pcna (assay MGI:5509378).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: retina |
Present |
Regionally restricted |
6E
,
6E'''
,
6E'
|
At E14.5, many more expressing cells became postmitotic.
|
|