Results
|
Specimen
2A,A',A''':
embryonic day 12.5
(more )
Note: Double labeled: magenta - Onecut1; green - Onecut2 (assay MGI:5509317).
Specimen
2A,A',A''':
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: magenta - Onecut1; green - Onecut2 (assay MGI:5509317).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: retina |
Present |
Regionally restricted |
2A'
,
2A
,
2A'''
|
There was 100% overlap of expression with Oc1, but Oc2 was weaker in most cells of the neuroblast layer.
|
Specimen
2B,B',B''':
embryonic day 14.5
(more )
Note: Double labeled: magenta - Onecut1; green - Onecut2 (assay MGI:5509317).
Specimen
2B,B',B''':
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: magenta - Onecut1; green - Onecut2 (assay MGI:5509317).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: retina |
Present |
Regionally restricted |
2B'
,
2B
,
2B'''
|
All cells in the ganglion cell layer express both genes. There were more cells expressing Oc1 than Oc2 in the neuroblast layer, but essentially all Oc2-positive cells were also Oc1-positive.
|
Specimen
2C,C',C''':
embryonic day 16.5
(more )
Note: Double labeled: magenta - Onecut1; green - Onecut2 (assay MGI:5509317).
Specimen
2C,C',C''':
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: magenta - Onecut1; green - Onecut2 (assay MGI:5509317).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: retina |
Present |
Regionally restricted |
2C'''
,
2C
,
2C'
|
The overlap of Oc1 and Oc2 expression remained close to 100% in both the ganglion cell layer and the presumed horizontal cells.
|
Specimen
not shown P0:
postnatal day 0
(more )
Note: Also stained for Onecut2 (assay MGI:5509317).
Specimen
not shown P0:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
postnatal day 0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Also stained for Onecut2 (assay MGI:5509317).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: retina ganglion cell layer |
Present |
|
|
The overlap of Oc1 and Oc2 expression remained close to 100% in the ganglion cell layer.
|
TS27: retina layer |
Present |
Regionally restricted |
|
The overlap of Oc1 and Oc2 expression remained close to 100% in the presumed horizontal cells.
|
Specimen
not shown P5:
postnatal day 5
(more )
Note: Also stained for Onecut2 (assay MGI:5509317).
Specimen
not shown P5:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
postnatal day 5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Also stained for Onecut2 (assay MGI:5509317).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: retina ganglion cell layer |
Weak |
|
|
The overlap of Oc1 and Oc2 expression remained close to 100% in the ganglion cell layer, although expression gradually decreased.
|
TS28: retina layer |
Present |
Regionally restricted |
|
The overlap of Oc1 and Oc2 expression remained close to 100% in the presumed horizontal cells.
|
Specimen
not shown P16:
postnatal day 16
(more )
Note: Also stained for Onecut2 (assay MGI:5509317).
Specimen
not shown P16:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
postnatal day 16
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Also stained for Onecut2 (assay MGI:5509317).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: retina ganglion cell layer |
Weak |
|
|
The overlap of Oc1 and Oc2 expression remained close to 100% in the ganglion cell layer, although expression gradually decreased.
|
TS28: retina layer |
Present |
Regionally restricted |
|
The overlap of Oc1 and Oc2 expression remained close to 100% in the presumed horizontal cells.
|
Specimen
5B,C:
postnatal day 16
(more )
Note: Double labeled: magenta - Onecut1; green - Calb1 (assay MGI:5509356).
Specimen
5B,C:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
postnatal day 16
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: magenta - Onecut1; green - Calb1 (assay MGI:5509356).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: retina inner nuclear layer |
Present |
Regionally restricted |
5B
,
5C
|
Expression was detected in horizontal cells. There was complete overlap with the expression of the horizontal cell marker Calbindin.
|
Specimen
5E,F:
postnatal day 16
(more )
Note: Double labeled: magenta - Onecut1; green - Lhx1 (assay MGI:5509361).
Specimen
5E,F:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
postnatal day 16
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: magenta - Onecut1; green - Lhx1 (assay MGI:5509361).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: retina inner nuclear layer |
Present |
Regionally restricted |
5E
,
5F
|
Expression was detected in horizontal cells. There was complete overlap with the expression of the horizontal cell marker Lim1.
|
Specimen
5M,M',M''':
embryonic day 14.5
(more )
Note: Double labeled: magenta - Onecut1; green - Lhx1 (assay MGI:5509361).
Specimen
5M,M',M''':
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: magenta - Onecut1; green - Lhx1 (assay MGI:5509361).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: retina |
Present |
Regionally restricted |
5M
,
5M'''
,
5M'
|
Expression was detected in Lim1-positive horizontal cell precursors.
|
Specimen
6A,A',A''':
embryonic day 12.5
(more )
Note: Double labeled: magenta - Onecut1; green - BrdU.
Specimen
6A,A',A''':
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: magenta - Onecut1; green - BrdU.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: retina |
Present |
Regionally restricted |
6A
,
6A'''
,
6A'
|
Expression was detected in the newly forming ganglion cell layer.
|
Specimen
6C,C',C''':
embryonic day 12.5
(more )
Note: Double labeled: magenta - Onecut1; green - phospho-histone H3.
Specimen
6C,C',C''':
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: magenta - Onecut1; green - phospho-histone H3.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: retina |
Present |
Regionally restricted |
6C'
,
6C'''
,
6C
|
Expression was detected in some M-phase cells.
|
Specimen
6D,D',D''':
embryonic day 14.5
(more )
Note: Double labeled: magenta - Onecut1; green - BrdU.
Specimen
6D,D',D''':
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: magenta - Onecut1; green - BrdU.
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: retina |
Present |
Regionally restricted |
6D'''
,
6D'
,
6D
|
At E14.5, many more expressing cells became postmitotic.
|
Specimen
6F,F',F''':
embryonic day 14.5
(more )
Note: Double labeled: magenta - Onecut1; green - phospho-histone H3.
Specimen
6F,F',F''':
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: magenta - Onecut1; green - phospho-histone H3.
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: retina |
Present |
Regionally restricted |
6F'
,
6F
,
6F'''
|
At E14.5, many more expressing cells became postmitotic.
|
Specimen
8A:
embryonic day 14.5; Atoh7tm1Gan/Atoh7 +
(more )
Specimen
8A:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Atoh7tm1Gan/Atoh7+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: retina |
Strong |
Regionally restricted |
8A
|
Expression was detected in a narrow band in the inner most regions of the retina.
|
Specimen
8B:
embryonic day 14.5; Pou4f2tm2Whk/Pou4f2 +
(more )
Specimen
8B:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Pou4f2tm2Whk/Pou4f2+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
8C:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6NCr
|
Mutant Allele(s):
|
Isl1tm1.2Whk/Isl1+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
8D:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Atoh7tm1Gan/Atoh7tm1Gan
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: retina |
Strong |
Regionally restricted |
8D
|
Expression was detected in a narrow band in the inner most regions of the retina as in wild type. There was no change in the level or pattern of expression in the mutant.
|
Specimen
8E:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Pou4f2tm2Whk/Pou4f2tm2Whk
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: retina |
Present |
|
8E
|
There was no change in the expression pattern or level in the mutant compared to the control.
|
Specimen
8F:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6NCr
|
Mutant Allele(s):
|
Isl1tm1.2Whk/Isl1tm1.2Whk
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: retina |
Present |
|
8F
|
There was no change in the expression pattern or level in the mutant compared to the control.
|
|