About   Help   FAQ
Myf5tm1.1(cre/Esr1*)Trdo
Targeted Allele Detail
Summary
Symbol: Myf5tm1.1(cre/Esr1*)Trdo
Name: myogenic factor 5; targeted mutation 1.1, Thomas A Rando
MGI ID: MGI:5509340
Synonyms: Myf5CreER-NN
Gene: Myf5  Location: Chr10:107318769-107321995 bp, - strand  Genetic Position: Chr10, 55.95 cM
Alliance: Myf5tm1.1(cre/Esr1*)Trdo page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:199482
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Inducible, Recombinase)
Inducer:    tamoxifen
Mutations:    Insertion, Intragenic deletion
 
Myf5tm1.1(cre/Esr1*)Trdo expression driven by 1 gene
 
Mutation details: A targeting vector was designed to place an internal ribosome entry site (IRES) fused to a CreERTM fusion gene (CreERTM; Cre recombinase fused to a G525R mutant form of the mouse estrogen receptor ligand binding domain) downstream of the stop codon in exon 3 of the Myf5 gene. A neomycin resistance marker, flanked by frt sites, was inserted downstream of the IRES-CreERTM cassette. This construct was electroporated into R1 embryonic stem cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were bred with C57BL/6 females. To remove the neo marker, mice were bred to B6.129S4-Gt(ROSA)26Sor/RainJ mice. (J:199482)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Myf5 (mouse)
Summary of all recombinase alleles driven by Myf5.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Myf5 Mutation:  17 strains or lines available
References
Original:  J:199482 Biressi S, et al., Myf5 expression during fetal myogenesis defines the developmental progenitors of adult satellite cells. Dev Biol. 2013 Jul 15;379(2):195-207
All:  3 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
11/19/2024
MGI 6.24 		The Jackson Laboratory