Trp53<tm1Thst> Targeted Allele Detail MGI Mouse (MGI:5518935)

Trp53^tm1Thst
Targeted Allele Detail

Summary

Symbol:	Trp53^tm1Thst
Name:	transformation related protein 53; targeted mutation 1, Thorsten Stiewe
MGI ID:	MGI:5518935
Synonyms:	p53^LSL-RR, Trp53^LSL-E177R
Gene:	Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
Alliance:	Trp53^tm1Thst page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:198482
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation
description

Allele Type:		Targeted (Conditional ready, Null/knockout)
Mutations:		Insertion, Nucleotide substitutions
		Mutation details: Exon 5 was replaced with a modified one in which nucleotide substitutions (GA-to-CG) result in the amino acid substitution of glutamic acid with arginine at position 177 (p.E177R), mimicking a mutation found in some human tumors. A loxP site flanked puromycin resistance gene cassette was inserted into intron 1. Authors state that this is a null allele. (J:198482)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

Tumor Data

List all tumor models in MMHCdb carrying Trp53^tm1Thst

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available Cell Lines: 0 lines available
Carrying any Trp53 Mutation:	240 strains or lines available

References

Original:	J:198482 Timofeev O, et al., p53 DNA binding cooperativity is essential for apoptosis and tumor suppression in vivo. Cell Rep. 2013 May 30;3(5):1512-25
All:	2 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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