Col1a1<tm1(CAG-Ezh2*)Meln> Targeted Allele Detail MGI Mouse (MGI:5519911)

Col1a1^{tm1(CAG-Ezh2*)Meln}
Targeted Allele Detail

Summary

Symbol:	Col1a1^{tm1(CAG-Ezh2*)Meln}
Name:	collagen, type I, alpha 1; targeted mutation 1, Ari Melnick
MGI ID:	MGI:5519911
Synonyms:	Ezh2^Y641N, Ezh2Y641N
Gene:	Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance:	Col1a1^{tm1(CAG-Ezh2*)Meln} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:199122
Parent Cell Line:	KH2 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Mutation
description

Allele Type:

Targeted (Conditional ready, Inserted expressed sequence, No functional change, RMCE-ready)

Mutation:

Insertion

Col1a1^{tm1(CAG-Ezh2*)Meln} expresses 1 gene

Mutation details: RMCE on KH2 cells inserted a cassette into the 3'UTR that contains a CAG promoter, a STOP/neomycin resistance cassette and cDNA for Ezh2 modified by nucleotide substitution (TAC to AAC) to contain the amino acid substitution asparagine for tyrosine at position 641 (Y641N). (J:199122)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:	163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae}. This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

Original:	J:199122 Beguelin W, et al., EZH2 is required for germinal center formation and somatic EZH2 mutations promote lymphoid transformation. Cancer Cell. 2013 May 13;23(5):677-92
All:	3 reference(s)

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