Gene Expression Data
Assay Details
Assay
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Reference:
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J:134646
Boekhoorn K, et al., Doublecortin (DCX) and doublecortin-like (DCL) are differentially expressed in the early but not late stages of murine neocortical development. J Comp Neurol. 2008 Apr 1;507(4):1639-52
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5521563
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Gene symbol:
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Vim
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Gene name:
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vimentin
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Antibody:
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anti-Vimentin
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Sections were post-fixed in Zamboni-mix (2% PFA/0.1% picric acid/0.025% glutaraldehyde/0.1M phosphate buffer, pH7.4).
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Results
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Specimen
1C:
embryonic day 9.0
(more )
Specimen
1C:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS14: prosencephalon |
Present |
Regionally restricted |
1C
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Expression is in radial gilal cells. Expression is in the ventricular zone, the inner wall of the third ventricle, and neocortical neuroepithelium.
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TS14: future diencephalon |
Present |
Regionally restricted |
1C
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Expression is in dorsal and ventral diencephalon including the thalamus and hypothalamus.
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Specimen
1H:
embryonic day 9.0
(more )
Specimen
1H:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS14: prosencephalon |
Present |
Regionally restricted |
1H
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Expression is in the ventricular zone and preplate and in a radial fibers crossing the intermediate zone.
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Specimen
2C:
embryonic day 10.0
(more )
Specimen
2C:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS16: telencephalon |
Present |
Regionally restricted |
2C
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Expression is in the lateral and medial parts.
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Specimen
2I:
embryonic day 10.0
(more )
Specimen
2I:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
2J:
embryonic day 10.0
(more )
Specimen
2J:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
4I:
embryonic day 11.0
(more )
Specimen
4I:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
5C:
embryonic day 13.0
(more )
Specimen
5C:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
5F:
embryonic day 13.0
(more )
Specimen
5F:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
not shown:
embryonic day 13.0
(more )
Specimen
not shown:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Regionally restricted |
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Expression is in the cellular profiles of the lower lumbal regions of the spinal cord.
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TS21: cerebellum |
Present |
Regionally restricted |
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Expression is in a radial fiber pattern.
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TS21: corpus striatum |
Present |
Regionally restricted |
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Expression is in a radial fiber pattern.
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TS21: telencephalon |
Present |
Regionally restricted |
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Expression is in a radial fiber pattern.
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Specimen
7C:
embryonic day 15.0
(more )
Specimen
7C:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 15.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
7H:
embryonic day 17.0
(more )
Specimen
7H:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 17.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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