Calb1<tm1.1(folA/cre)Hze> Targeted Allele Detail MGI Mouse (MGI:5522769)

Calb1^{tm1.1(folA/cre)Hze}
Targeted Allele Detail

Summary

Symbol:	Calb1^{tm1.1(folA/cre)Hze}
Name:	calbindin 1; targeted mutation 1.1, Hongkui Zeng
MGI ID:	MGI:5522769
Synonyms:	Calb1-2A-dgCre-D, Calb1-2A-dgCre-Delta, Calb1-2A-dgCre-DeltaNeo, Calb1-2A-DHFR-EGFP-Cre-D, Calb1-2A-ecDHFR^R12Y/Y100I/EGFP/Cre-D, Calb1^(dgCre)Hze, Calb1-T2A-dgCre-D
Gene:	Calb1 Location: Chr4:15881264-15906709 bp, + strand Genetic Position: Chr4, 6.66 cM
Alliance:	Calb1^{tm1.1(folA/cre)Hze} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:243762
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Mutation
description

Allele Type:

Targeted (Inducible, Recombinase)

Inducer:

trimethoprim

Mutation:

Insertion

Calb1^{tm1.1(folA/cre)Hze} expression driven by 1 gene

Mutation details: The targeting vector inserted a viral 2A oligopeptide (T2A, mediating ribosomal skipping) and a destabilized EGFP/Cre fusion gene (dgCre) inserted immediately downstream of the calbindin 1 translational STOP codon. The dgCre fusion gene (also called destabilized EGFP/Cre, DHFR-EGFP-Cre, hDHFR/EGFP/Cre or ecDHFR/EGFP/Cre) is an enhanced green fluorescent protein/Cre recombinase fusion gene with an N terminal fusion of the first 159 amino acids of the Escherichia coli K-12 strain chromosomal dihydrofolate reductase gene (DHFR or folA) harboring the G67S mutation and modified to also include the R12Y/Y100I destabilizing domain mutations. A PGK-neo-polyA cassette flanked by AttB and AttP sites was also part of the construct. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were bred to PhiC31-expressing mice to delete the AttB/AttP-flanked sequences and replace it with the recombined AttB/AttP site (AttL). Trimethoprim-treatment is required to stabilize the cre protein. No EGFP is detected with or without induction. (J:243762)

Recombinase
activity

Activity:	Tissue activity of this recombinase allele
Driver:	Calb1 (mouse) Summary of all recombinase alleles driven by Calb1. Comparative matrix view of recombinase activities Alliance mouse Calb1 gene page

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Calb1 Mutation:	28 strains or lines available

References

Original:	J:243762 Evans RC, et al., Dopamine Inhibition Differentially Controls Excitability of Substantia Nigra Dopamine Neuron Subpopulations through T-Type Calcium Channels. J Neurosci. 2017 Mar 29;37(13):3704-3720
All:	18 reference(s)

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