Tg(Gcg-icre)23Cshi
Transgene Detail
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| Symbol: |
Tg(Gcg-icre)23Cshi |
| Name: |
transgene insertion 23, Chiyo Shiota |
| MGI ID: |
MGI:5529611 |
| Synonyms: |
Gcg-Cre |
| Transgene: |
Tg(Gcg-icre)23Cshi Location: unknown
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| Alliance: |
Tg(Gcg-icre)23Cshi page
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| Transgene Type: |
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Transgenic (Recombinase) |
| Mutation: |
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Insertion
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Tg(Gcg-icre)23Cshi expression driven by
1 gene
Transgene expression driven by:
| Organism |
Driver Gene |
Note |
| Not Specified |
Gcg |
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Mutation details: BAC recombineerting was used to generate a construct with a codon-improved cre recombinase sequence inserted into the translation initiation site in exon 2 of the glucagon (Gcg) gene, followed by n adenylation signal sequence. The large first intron of Gcg was replaced with a 300 bp synthetic intron to reduce the size of the final transgene construct that was injected into pronuclei of C57BL/6 zygotes. Founders were screened by PCR for transgene intregration, with 20 and 23 testing positive for integration. Line 23 was chosen for further analysis.
(J:203933)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Gcg
(species not specified)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:203933 Shiota C, et al., alpha-Cells are dispensable in postnatal morphogenesis and maturation of mouse pancreatic islets. Am J Physiol Endocrinol Metab. 2013 Oct 15;305(8):E1030-40 |
| All: |
4 reference(s) |
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Analysis Tools
