Hprt1tm331a(Ple275-icre/ERT2)Ems
Targeted Allele Detail
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| Symbol: |
Hprt1tm331a(Ple275-icre/ERT2)Ems |
| Name: |
hypoxanthine phosphoribosyltransferase 1; targeted mutation 331a, Elizabeth M Simpson |
| MGI ID: |
MGI:5529935 |
| Synonyms: |
GABRA6-creERT2, Ple275-icre/ERT2;mEMS5742 |
| Gene: |
Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
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| Alliance: |
Hprt1tm331a(Ple275-icre/ERT2)Ems page
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| Mutant Cell Line: |
mEMS5742 |
| Germline Transmission: |
Earliest citation of germline transmission:
J:204202
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| Parent Cell Line: |
mEMS4857.02 (ES Cell)
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| Strain of Origin: |
B6N.Cg-Aw-J Hprt1b-m3
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| Project Collection: |
EUCOMMTOOLS
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| Allele Type: |
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Targeted (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Hprt1tm331a(Ple275-icre/ERT2)Ems expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Homolog in Mouse |
Note |
| human |
GABRA6 (2559) |
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Mutation details: The transgene consists of a RP11-631.F18 human bacterial artificial chromosome (BAC) retrofitted to contain a human HPRT complementary sequence (containing exon 1, intron 1, exon 2 and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm; all inserted into the sacB gene in the BAC vector. Next, an iCre/ERT2 fusion gene, an intron, an SV40 early polyA signal and an Frt-flanked kanamycin resistance (Kan) cassette were inserted directly at the ATG of the human GABRA6 locus. Transient transfection with FLP recombinase removed the Kan cassette before the modified BAC was used for targeting as a single copy "knock-in" into the upstream region of the Hprt locus.
(J:204202)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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GABRA6
(human)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Hprt1 Mutation: |
1279 strains or lines available
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| Original: |
J:204202 The Pleiades Promoter and CanEuCre Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2014; |
| All: |
1 reference(s) |
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Analysis Tools
